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BOND-III Fully Automated IHC and ISH Staining System


Make a Difference with Accurate and Timely Slides

The BOND-III stainer provides you with all of the pieces to solve the diagnostic puzzle quickly and consistently. Maximize your lab space with this compact solution that combines efficiency and organization, allowing you to achieve up to 62% more slide throughput per m2* compared to other leading instruments on the market.

  • Independently benchmarked antibody clones means quality that Pathologists can trust: pair the BOND-III stainer with optimized Novocastra HD BOND reagents and BOND Compact Polymer Detection solutions.
  • Preserve morphology and integrity of precious tissue with the unique Covertile system that allows you to dispense reagents in a highly-controlled and more consistent manner. 91% of customers find the stain quality and reproducibility to be better than the competition.*
  • Designed with the user in mind, everything from immediate reagent access with real-time measurements/alerts and greater flexibility over the competition to swap out reagents during protocol runs, to the intuitive user interface and case management with 3 independent trays, makes the BOND-III instrument the ultimate team player to meet your lab’s needs.
Contact us to get a quote. Call +1 844 534 2262 to speak with a representative.

*Independent Studies
For In Vitro Diagnostic Use

Product Features

When Delivering a Case to the Pathologist, it’s the Last Slide that Matters

A complete case means that the last slide is as important as the first. The BOND-III stainer helps you deliver the entire case in the shortest amount of time while maintaining excellent standards in quality. Other systems prioritize individual slides at the expense of overall case completion, meaning it takes longer to finalize the last slide in the case.

“Diagnosing a case without all of the slides is like trying to do a puzzle with pieces missing…”

Interested in how the BOND-III stainer compares against the Ventana BenchMark ULTRA in turnaround time?

Get the White Paper

Last Slide Matters

Make Every Inch of Your Lab Count

Meet the demands of ever-increasing slide volumes with the space you have. The BOND-III stainer produces the most number of slides in the least amount of space, allowing you to achieve up to 62% more throughput per m2 compared to other leading instruments.


Your Next Step Illuminated

Never worry about when a bottle needs to be filled or emptied. The BOND-III stainer is the only IHC/ISH instrument to illuminate bulk reagent levels with LED backlighting to provide a clear, visual alert should the laboratory need to either top-up a reagent bottle, or empty a waste bottle.

BOND-III Illuminates Reagent Levels

Designed with the User in Mind

The highly visual user interface features easy-to-identify icons, making it faster, easier, and more efficient for staff to execute tasks and avoid errors. In addition, with the BOND-III platform you have immediate access to antibodiesprobes, and detection systems with real-time measurements, alerts for reagent management, and greater flexibility over the competition to swap out reagents during protocol runs.

BOND-III User Friendly Interface

Covertile System – Reduce Waste

The BOND Covertile minimizes the amount of reagent needed to make an IHC slide. This generates low total waste volumes while the BOND-III instrument additionally separates hazardous waste from standard waste for efficient waste disposal.

Covertile System – Ensure Quality

The BOND Covertile provides total tissue care throughout the entire staining process. The BOND Covertile protects tissue morphology, allows full tissue coverage, and enables gentle and consistent reagent application.

Proven Staining Quality = Quality Pathologists can Trust

With in-house development of our antibodies and the complete BOND system – instrument, antibodies, diluent, and detection working together in an optimized manner – we ensure superior real-world performance.

Our Novocastra reagent solutions include fully-automated and ready-to-use (RTU) antibodies, probes, detection systems, and ancillaries, empowering labs to deliver high-quality and reliable staining to support patient care.

“The basis of all rational therapy is the diagnosis…”

BOND RTU antibodies and detection systems

Novocastra HD Antibodies Deliver Higher Optimal Staining Rates Compared to Leading Vendors

To deliver the performance you demand, our Novocastra HD line is independently evaluated. Head-to-head comparisons against other leading vendors show that our antibodies consistently deliver superior staining quality.

Novocastra HD Antibodies Comparison

Exceptional Sensitivity for Exceptional Detection with Compact Polymer Technology

  • Lean efficiency – full automation reduces waste and process variation
  • Complete system – all aspects of the system optimized to work together to support your entire process

Product Specifications

Dimensions & Weight

Dimensions (W x H x D): 790 mm x 1378 mm x 806 mm
(31.1 x 54.25 x 31.73 in)
Weight (Dry): 238 kg (525 lbs)


Operating Voltage (older-model power supply; one fan in back cover): 103.4 V to 127.2 V (for nominal voltage 110 V to 120 V)
Power Consumption: 1200 VA
Operating Voltage (newer-model power supply; two fans in back cover): 90 V to 264 V (for nominal voltage 100 V to 240 V)
Mains Frequency: 50/60 Hz

Environmental (Unpacked instruments)

Maximum Operating Temperature: 35°C (95°F)
Minimum Operating Temperature: 5°C (41°F)
Temperature Required to meet Staining Performance Requirements: 18–26°C (64–79°F)
Maximum Heating Energy Output: 1200W/1000W
Operating Humidity (Non-Condensing): 30 to 80% RH
Maximum Operating Altitude: 0 to 1600 m (5250 ft.) above sea level
Sound Pressure Level Output (at 1 m): < 85 dBA maximum
< 65 dBA normal operation


Slide Capacity: 30
Finished trays (10 slides) may be replaced continuously
Number of Reagent Containers: 36
Reagent Container Capacity: 7 mL and 30 mL
Titration Container Capacity: 6 mL
Bulk Reagent Container Capacity: 2 L or 5 L
Hazardous Waste Container Capacity: 5 L
Standard Waste Container Capacity: 2 x 5 L
Chemical Compatibility: All BOND Reagents
70% Alcohol Solution (for cleaning purposes)
Temperature Indication: Defaults (these can be changed by service representatives): Warm: 35°C, Hot: 80°C
Maximum permitted pressure for gas and liquid connections: 1.0 bar

Microscope Slides

Slide Width: 24.64–26.0 mm (0.97–1.02 in)
Slide Length: 74.9–76.0 mm (2.95–2.99 in)
Slide Thickness: 0.8–1.3 mm (0.03–0.05 in)
Label Area Width: 24.64–26.0 mm (0.97–1.02 in)
Label Area Length: 16.9–21.0 mm (0.67–0.83 in)
Material: Glass, ISO 8037/1


Storage Temperature: -20 to 55 °C (-4 to 131 °F)
Storage Humidity (Non-Condensing): < 80% RH
Shipping Methods: Road and Air Freight Compatible




BOND RTU Antibody and Protocol Data Files




BOND RTU Reagents and Protocol Data Files

These data files will enable the use of the latest releases from the BOND RTU range. Local licensing restrictions may apply to some products; please contact your local Leica Biosystems Sales or Support representative should you have any queries. Please ensure the correct data file is downloaded.


Important Notes

  • BDDv94 is only compatible with BOND software v5 or higher
  • BOND software v4.0 BDZ files are no longer supported after BDZ v74
  • Do not apply this update to BOND RX or BOND RXm research systems.
  • Ensure the correct update is applied commensurate with your software version and region.
  • We recommend that all BOND systems be updated to the latest software version
BOND-III Interactive Product Demo