Sacrifice Perfusion System Controlled Pressure
The Perfusion Two Automated Pressure Perfusion system includes an automated air compressor that quietly pumps the air tank of the Perfusion One up to 300 mm Hg and stops. As each animal is being prepared, the pressure is set up to begin at the turn of a switch.
Pressure above physiological pressure is required to clear all blood vessels and leave no red blood cells. Automating the perfusion system gives much greater consistency and more reproducable perfusions, as well as greater convenience and ease of operation.
Pressure washout gets all blood cells out in about 5 seconds. Fixative can get in sooner.
Better HRP or Fluorescence Results
Removing all red bloods cells reduces HRP background and autofluoroesence
Better Tissue Quality
Any red blood cells left block capillaries, and thus access of fixative to tissue downstream. Fast removal of all of them yields more even fixation.
Avoid Soft Tissue Shrinkage
With proper management of fixative tonicity, discussed in the references, you can avoid tissue shrinkage.