Leica CM3050 S Research Cryostat

Primarily designed for the demanding needs of cryosectioning in biomedical, neuro-anatomical and pharmaceutical research

The Leica CM3050 S cryostat features superior user comfort with excellent safety standards for practically all types of cryosectioning applications.

It is the instrument of choice for all research applications and for advanced clinical cryosectioning needs.

Particularly when working with delicate specimens – for example brain samples in neuroscience – the precise specimen orientation and the specimen feed system guarantees reproducible, thin, serial sections of maximum quality.

Select a configuration

View Configurations

Specs

Technical Specifications

General

Width (with handwheel):	835 mm
Depth (cabinet only):	850 mm
Height:	1215 mm
Working height (armrest):	1025 mm
Weight (w/ motor and suct.):	193 kg
Operating temperature range:	18°C to 35°C
Maximum specimen size:	50 x 80 mm
Cutting speed:	Slow: 0-50 strokes/min, Fast: 0-85 strokes/min, Vmax: 85-90 strokes/min
Section thickness setting	0.5 to 300 μm
Maximum specimen size	40 mm x 55 mm
Horizontal specimen feed	25 mm
Vertical specimen stroke	59 mm
Specimen retraction	50 pm

Specimen precision orientation	by 8° (x/y/z axis)
Trimming	5 to 150 μm ± 0,5 μm in steps of 5, 10, 30, 50, 100 and 150 μm
Motorized coarse feed at two speeds	500 μm/s and 1,000 μm/s
Cutting speed ranges	0.1 mm/s to 170 mm/s, 0.1 mm/s to 100 mm/s, Vmax 210 mm/s
Temperature setting range	0°C to -40°C
Defrosting	programmable 1 automatic defrost cycle/24 h duration; from 6 to 12 min; manual defrosting
Freezing shelf temperature	Approx. -43°C at an ambient temperature of 22°C
Temperature setting range	-10°C to -50°C (+/-3 K)
Dimensions (W x H x D)	882 x 1040 x 766 mm
Weight (including microtome)	Approx. 180 kg
Power draw	1800 VA

Features

Product Features

Precise stepper motor

Exceptionally precise stepper motor promotes the production of high quality sections of consistent thickness.

Fine orientation control

Fine orientation control supports sectioning of delicate tissues.

Optional motorized sectioning

Optional motorized sectioning provides reproducible section quality and thickness and reduces repetitive motion.

Programmable reverse counter

Programmable reverse counter saves time by promoting fast trimming between levels (step sectioning).

Section thickness totalizer

Section thickness totalizer with re-set button facilitates target preparation.

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Documents

Application Notes

Application Paper Cryoembedding EN

(PDF)

Brochures

95.8846 Rev D CM3050 S Brochure EN	(PDF)
95.8847 Rev D CM3050 S Brochure DE	(PDF)
95.8848 Rev D CM3050 S Brochure FR	(PDF)
95.8849 Rev D CM3050 S Brochure ES	(PDF)
95.8850 Rev D CM3050 S Brochure IT	(PDF)

Certificates

EU Declaration Leica CM3050s A en

(PDF)

Dedicated Articles

pathologypartners

(PDF)

Material Safety Data Sheets

Cryostat oil Typ Isoflex PDP 38 2015-04-02 10695-0007 CHN-zh	(PDF)
Cryostat oil Typ Isoflex PDP 38 2015-04-02 10695-0007 D-de	(PDF)
Cryostat oil Typ Isoflex PDP 38 50ml 14033606098 Rev.01 GB	(PDF)
Kühlöl Typ Isoflex PDP 38 E-es 10695-0007 1 0	(PDF)
Kühlöl Typ Isoflex PDP 38 F-fr 10695-0007 1 0	(PDF)
Kühlöl Typ Isoflex PDP 38 I-it 10695-0007 1 0	(PDF)
Leica Cryofect E-es 10695-0009 1 2	(PDF)
Leica Cryofect F-fr 10695-0009 1 2	(PDF)
Leica Cryofect I-it 10695-0009 1 2	(PDF)

Preventive Maintenance

Disinfection Protocol for Cryostats Leica

(PDF)

Publications

Face Down Cryoembedding	(PDF)
pathoplogypartners dr.peters	(PDF)

User Manuals/IFUs

Leica CM3050S IFU 2v0G cs	(PDF)
Leica CM3050S IFU 2v0G da	(PDF)
Leica CM3050S IFU 2v0G de	(PDF)
Leica CM3050S IFU 2v0G el	(PDF)
Leica CM3050S IFU 2v0G en	(PDF)
Leica CM3050S IFU 2v0G es	(PDF)
Leica CM3050S IFU 2v0G fr	(PDF)
Leica CM3050S IFU 2v0G hr	(PDF)
Leica CM3050S IFU 2v0G it	(PDF)
Leica CM3050S IFU 2v0G ja	(PDF)
Leica CM3050S IFU 2v0G lt	(PDF)
Leica CM3050S IFU 2v0G nl	(PDF)
Leica CM3050S IFU 2v0G no	(PDF)
Leica CM3050S IFU 2v0G pl	(PDF)
Leica CM3050S IFU 2v0G pt	(PDF)
Leica CM3050S IFU 2v0G ru	(PDF)
Leica CM3050S IFU 2v0G sk	(PDF)
Leica CM3050S IFU 2v0G sv	(PDF)
Leica CM3050S IFU 2v0G tr	(PDF)
Leica CM3050S IFU 2v0I zh	(PDF)
Leica MH 2v3C bg	(PDF)
Leica MH 2v3C cs	(PDF)
Leica MH 2v3C da	(PDF)
Leica MH 2v3C de	(PDF)
Leica MH 2v3C el	(PDF)
Leica MH 2v3C en	(PDF)
Leica MH 2v3C es	(PDF)
Leica MH 2v3C fr	(PDF)
Leica MH 2v3C hr	(PDF)
Leica MH 2v3C hu	(PDF)
Leica MH 2v3C it	(PDF)
Leica MH 2v3C ja	(PDF)
Leica MH 2v3C lt	(PDF)
Leica MH 2v3C nl	(PDF)
Leica MH 2v3C no	(PDF)
Leica MH 2v3C pl	(PDF)
Leica MH 2v3C pt	(PDF)
Leica MH 2v3C ro	(PDF)
Leica MH 2v3C ru	(PDF)
Leica MH 2v3C sk	(PDF)
Leica MH 2v3C sr	(PDF)
Leica MH 2v3C sv	(PDF)
Leica MH 2v3C tr	(PDF)
Leica MH 2v3C zh	(PDF)

Resources

Configurations

Choose your option below, and then use the Contact Us link above to submit a quote request for your selection

Configuration 1 (14903050S01)

Freestanding, motorized open-top cryostat with independent specimen temperature control.
Spacious, easy-to-clean cryochamber with illumination.
Removable heated glass sliding window.
Cryochamber temperature setting down to -40 °C in 1° steps.
Permanently cooled (down to -45 °C) quick-freeze shelf for 10 specimen discs.

Configuration 2 (14903050S02)

Leica CM3050 S Configuration 120 V / 60 Hz With sectioning motor and with specimen temperature control with knife holder CN
Freestanding, motorized open-top cryostat with independent specimen temperature control.
Spacious, easy-to-clean cryochamber with illumination.
Removable heated glass sliding window.
Cryochamber temperature setting down to -40 °C in 1°increments.
Permanently cooled (down to -45 °C) quick-freezeshelf for 10 specimen discs.

Basic Instrument (14903050S03)

Leica CM3050 S Configuration 120 V / 60 Hz with sectioning motor, without specimen temperature control
Freestanding, motorized open-top cryostat without independent specimen temperature control.
Spacious, easy-to-clean cryochamber with illumination.
Removable heated glass sliding window.
Cryochamber temperature setting down to -40 °C in 1° steps.

Consumables

Knife holders and Knife holder base

Code	Name Show all details	Qty
14041926140	Knife holder base for CN, CE, CS Knife holder base for CN and CE holder
14041933993	Knife holder CN w/o knife holder base Knife holder CN: Knife holder CN for reusable microtome knives or magnetic blade rails. Clearance angle setting and knife height adjustment. Knife support rails for short and long knives. Anti-roll guide and adjustable knife guard.
14041933990	Knife holder CE low prof. Knife holder CE: Knife holder CE for low profile disposable blades with lateral displacement feature and glass anti-roll guide. Clearance angle setting.
14041933991	Knife holder CE high prof. Knife holder CE: Knife holder CE for high profile disposable blades, with lateral displacement feature and glass anti-roll guide. Clearance angle setting.
14041933992	Blade holder CE Blade holder CE for low profile disposable blades, w/o clearance angle adjustment, including knife holder base and pressure plates.
14041932073	Knife holder CE-TC Knife holder CE-TC for Tungsten Carbide disposable blades. Especially suitable for sectioning harder specimens, such as tissue with bone or cartilage.
14041935693	Anti-roll guide, assy. 70mm, tilting Retrofit kit: tilting device with anti-roll system CE Consisting off: tilting device anti-roll system CE: glass insert 70 mm metal frame CE 100 µm spacer between knife holder, pressure plate and anti-roll system, for standard applications Sections between 5 µm - 50 µm For knife holders CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14041933980	Anti-Roll Plate, Assy. 70mm 100µm Anti-roll system CE, glass - 70 mm For standard application Consisting off: glass insert 70 mm metal frame CE 100 µm spacer between knife holder, pressure plate and anti-roll system, for standard applications sections between 5 µm-50 µm For knife holder CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14041933981	Anti-roll plate,assy. glass 50mm Anti-roll system for knife holder CN or knife holder CNZ, consisting of: 50 mm wide glass insert, metal frame CN, CNZ for exchangeable glass inserts sections between 5 µm-50 µm
14041937258	Anti-roll plate, assy. 70mm 50µm Anti-roll system CE, glass - 70 mm For special application Consisting off: glass insert 70 mm metal frame CE 50 µm spacer between knife holder, pressure plate and anti-roll system, for special applications sections below 4 µm For knife holder CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14041937260	Anti-roll plate, assy. 70mm 150µm Anti-roll system CE, glass - 70 mm For special application Consisting off: glass insert 70 mm metal frame CE 150 µm spacer between knife holder, pressure plate and anti-roll system, for special applications sections above 50 µm For knife holder CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14047742497	Glass Insert 70 mm pol. Glare-minimizing glass insert, 70 mm wide; replacement glass for anti-roll systems CE (14 0477 42491, 14 0477 42492, 14 0477 42493) for blade holder CE (14 0477 43005)
14041933816	Anti-roll plate, glass 50mm Glass insert - 50 mm For knife holder CN (14 0477 42358)
14050229553	Pressure Plate Rear BB, 22° Pressure plate B, 22 °, for high profile blades
14050229551	Pressure plate rear SB, 22° Pressure plate S, 22 °, for low profile blades
14080037740	Anti-static kit high-pr. Anti-static kit for knife holder CE for high profile disposable blades
14080037739	Anti-static kit low-prof. Anti-static kit for knife holder CE for low profile disposable blades
14041919426	Knife support Knife support bar for knife holder CN for short knives
14041919427	Knife support Knife support bar for knife holder CN for long knives

Disposable blades

Code	Name Show all details	Qty
14021626379	Disposable blades TC-65 1Package=5Blades Leica TC65 Microtome Blade System for hard Material sectioning The Leica TC65 tungsten carbide disposable blades are specially designed and manu- factured to meet the stringent require- ements of laboratories routinely sec- tioning hard and abrasive materials. The unique cutting edge treatment guarantees sections down to approx.1µm. The blades are fully recyclable. length: 65 mm thickness: 1 mm height: 11 mm Leica TC65 tungsten carbide disposable blades. Box of 5.
14035838926	High-profile disposable blades 818(1x50) Leica Disposable Blades, high profile (818) 80 mm long, 14 mm high 1 Package of 50 blades
14035838383	High-profile disposable blades818(10x50) Leica Disposable Blades, high profile (818) 80 mm long, 14 mm high 10 Packages of 50 blades each
14035838925	Low-profile disposable blades 819 (1x50) Leica Disposable Blades, low profile (819) 80 mm long, 8 mm high 1 Package of 50 blades
14035838382	Low-profile disposable blades 819(10x50) Leica Disposable Blades, low profile (819) 80 mm long, 8 mm high 10 Packages of 50 blades each
14035843488	Low-profile disp.blades DB80LS (1x50) Leica Disposable Blades, low profile - SB DB80 LS for standard applications 1 package of 50 blades
14035843489	Low-profile disp.blades DB80LS (10x50) Leica Disposable Blades, low profile - SB DB80 LS for standard applications 10 packages of 50 blades each
14035843498	Low-profile disp.blades DB80LS (1x10) Leica Disposable Blades, low profile - SB DB80 LS for standard applications 1 package of 10 blades
14035843490	High-profile disp.blades DB80HS (1x50) Leica Disposable Blades, high profile - BB DB80 HS for standard applications 1 package of 50 blades
14035843491	Hig-profile disp.blades DB80HS (10x50) Leica Disposable Blades, high profile - BB DB80 HS for standard applications 10 packages of 50 blades each
14035843499	High-profile disp.blades DB80HS (1x10) Leica Disposable Blades, high profile - BB DB80 HS for standard applications 1 package of 10 blades
14035843496	Low-profile disp.blades DB80LX (1x50) Leica Disposable Blades, low profile - SB DB80 LX for standard applications (extra durable) 1 package of 50 blades
14035843497	Low-profile disp.blades DB80LX (10x50) Leica Disposable Blades, low profile - SB DB80 LX for standard applications (extra durable) 10 packages of 50 blades each
14035843502	Low profile disp.blades DB80LX (1x10) Leica Disposable Blades, low profile - SB DB80 LX for standard applications (extra durable) 1 package of 10 blades

Reusable knives

Material with code "14021607130" is discontinued

Code	Name Show all details	Qty
14021607100	Knife, 16 cm long, profile c steel assy Knife, 16 cm long, profile c (plane on both sides, for paraffin and frozen sections ) Note: Knife case 14 0213 11140 included
14021604206	Knife 16 cm c TC assy Knife, 16 cm TC edge, profile c Note: Knife case 14 0213 11140 included
14021607132	Knife, 16 cm long, profile d steel assy Knife, 16 cm long, profile d Note: Knife case 14 0213 11140 included
14021604813	Knife 16 cm d TC, assy Knife, 16 cm TC- edge, profile d Note: Knife case 14 0213 11140 included
14021626379	Disposable blades TC-65 1Package=5Blades Leica TC65 Microtome Blade System for hard Material sectioning The Leica TC65 tungsten carbide disposable blades are specially designed and manu- factured to meet the stringent require- ements of laboratories routinely sec- tioning hard and abrasive materials. The unique cutting edge treatment guarantees sections down to approx.1µm. The blades are fully recyclable. length: 65 mm thickness: 1 mm height: 11 mm Leica TC65 tungsten carbide disposable blades. Box of 5.

Dr.Peters Cryoembedding systems

Code	Name Show all details	Qty
14020139115	Cryoembed.system cpl.set Cryoembedding System, complete set consisting of: 3 well bars in three sizes: 18 mm, 24 mm, 30 mm 6 small stages 4 large stages 4 over-stage heat extractors 1 bin for stages 16 dispensing slides 1 cutting board/freezing griddle 1 elevated heat extractor 1 angled embedding forceps with accessories
14020139116	Embed. well bar set 18 mm Small Embedding Well Bar Set consisting of: 1 well bar, 18 mm 4 small stages 2 over-stage heat extractors 8 dispensing slides
14020139117	Embed. well bar set 24 mm Medium Embedding Well Bar Set consisting of: 1 well bar, 24 mm 4 small stages 2 over-stage heat extractors 8 dispensing slides
14020139118	Embed. well bar set 30 mm Large Embedding Well Bar Set consisting of: 1 well bar, 30 mm 4 large stages 2 over-stage heat extractors 8 dispensing slides
14020139119	Freez.griddle/block elev. Freezing Griddle / elevated heat extractor consisting of: 1 epoxy-coated forceps 1 putty knife extra elevating bars
14020139120	Embedding well bar 4x18mm Embedding Well Bar thickness: 1 inch size: 4 x 18 mm, depth 3 mm stainless steel 4 wells
14020139121	Embedding well bar 4x24mm Embedding Well Bar thickness: 1 inch size: 4 x 24 mm, depth 3 mm stainless steel 4 wells
14020139122	Embedding well bar 3x30mm Embedding Well Bar thickness: 1 inch size: 3 x 30 mm, depth 3 mm stainless steel 3 wells
14020139123	Spec. stage square 28 mm Specimen stage, square stainless steel small - 28 mm pack of 1
14020139124	Spec. stage square 36 mm Specimen stage, square stainless steel large - 36 mm pack of 1
14020139125	Ovewr-disc heat extractor Over-stage heat extractor
14020139126	Bin for discs Bin for stages
14020139127	Dispensing slides 8 pcs. Dispensing slides for Dr. Peter´s cryoembedding system pack of 8

Consumables

Code	Name Show all details	Qty
14041935693	Anti-roll guide, assy. 70mm, tilting Retrofit kit: tilting device with anti-roll system CE Consisting off: tilting device anti-roll system CE: glass insert 70 mm metal frame CE 100 µm spacer between knife holder, pressure plate and anti-roll system, for standard applications Sections between 5 µm - 50 µm For knife holders CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14041933980	Anti-Roll Plate, Assy. 70mm 100µm Anti-roll system CE, glass - 70 mm For standard application Consisting off: glass insert 70 mm metal frame CE 100 µm spacer between knife holder, pressure plate and anti-roll system, for standard applications sections between 5 µm-50 µm For knife holder CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14041933981	Anti-roll plate,assy. glass 50mm Anti-roll system for knife holder CN or knife holder CNZ, consisting of: 50 mm wide glass insert, metal frame CN, CNZ for exchangeable glass inserts sections between 5 µm-50 µm
14041937258	Anti-roll plate, assy. 70mm 50µm Anti-roll system CE, glass - 70 mm For special application Consisting off: glass insert 70 mm metal frame CE 50 µm spacer between knife holder, pressure plate and anti-roll system, for special applications sections below 4 µm For knife holder CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14041937260	Anti-roll plate, assy. 70mm 150µm Anti-roll system CE, glass - 70 mm For special application Consisting off: glass insert 70 mm metal frame CE 150 µm spacer between knife holder, pressure plate and anti-roll system, for special applications sections above 50 µm For knife holder CE: (14 0419 33990, 14 0419 33991, 14 0419 33992)
14047742497	Glass Insert 70 mm pol. Glare-minimizing glass insert, 70 mm wide; replacement glass for anti-roll systems CE (14 0477 42491, 14 0477 42492, 14 0477 42493) for blade holder CE (14 0477 43005)
14041933816	Anti-roll plate, glass 50mm Glass insert - 50 mm For knife holder CN (14 0477 42358)
14020108926	Tissue freezing medium, 125 ml Tissue freezing medium for cryosectioning 125 ml
14033606100	Cryostat oil type Isoflex PDP 38, 250ml Cryostat oil - type Isoflex PDP 38 bottle of 250 ml Shelf life 36 months
14034029011	Safety gloves size M Cut resistant safety gloves size M
14034040859	Safety gloves size S Cut resistant safety gloves size S

Basic instruments

Parts/Accessories

Accessories for Leica Cryoclean

Other Accessories

Code	Name Show all details	Qty
14047743247	O-ring blue (10pcs) O-ring blue, for color-marking of 20 mm and 30 mm diameter sepecimen disc (14 0477 40044) pack of 10
14047743248	O-ring red (10pcs) O-ring red, for color-marking of 20 mm and 30 mm diameter specimen disc (14 0477 40044) pack of 10
14047743249	O-ring blue (10pcs) O-ring blue, for color-marking of 40 mm diameter specimen disc (14 0477 40045) pack of 10
14047743250	O-ring red (10pcs) O-ring red, for color-marking of 40 mm diameter specimen disc (14 0477 40045) pack of 10
14047743251	O-ring blue (10pcs) O-ring blue, for color-marking of 55 mm diameter specimen disc (14 0477 40046) pack of 10
14047743252	O-ring red (10pcs) O-ring red, for color-marking of 55 mm diameter specimen disc (14 0477 40046) pack of 10
14043643463	Tool kit CM.. Tool kit for Cryostats, consisting of: 1 Brush, fine (14 0183 28642) 1 Leica brush with magnet (14 0183 40426) 1 Allen key with handle, size 5, metric (14 0194 04760) 1 Allen key, size 2.5, metric (14 0222 04137) 1 Allen key, size 3.0, metric (14 0222 04138) 1 Allen key, size 4.0, metric (14 0222 04139) 1 Allen key, size 5.0, metric (14 0222 04140) 1 Allen key, size 6.0, metric (14 0222 04141) 1 Allen key, size 1.5, metric (14 0222 10050) 1 Allen key, size 4.0, with spherical head (14 0222 32131) 1 Single-head wrench, size 16 (14 0330 18595) 1 Single-head wrench, size 13 (14 0330 33149)
14071240150	Easy Dip staining cnt. wt 64mmx76mmx92mm Easy Dip, Slide Staining Vessel white pack of 6
14071240151	Easy Dip staining cnt. pk 64mmx76mmx92mm Easy Dip, Slide Stainig Vessel pink pack of 6
14071240152	Easy Dip staining cnt.grn 64mmx76mmx92mm Easy Dip, Slide Staining Vessel green pack of 6
14071240153	Easy Dip staining cnt. yl 64mmx76mmx92mm Easy Dip, Slide Staining Vessel yellow pack of 6
14071240154	Easy Dip staining cnt. bl 64mmx76mmx92mm Easy Dip, Slide Staining Vessel blue pack of 6
14071240161	Easy Dip Stain. rack gray 60mmx64mmx97mm Easy Dip, Slide Staining Rack for 12 microscope slides gray pack of 6
14044326148	Height adj.module CM3050S Cabinet height adjustment module - electrohydraulic lifting device. Stable U-shape metal frame incl. control unit. Lifting height: 230 mm Dimensions: Width 880 mm excl. control unit - 1030 mm incl. control unit Weight: approx. 50 kg 100-120 V/60 Hz
14037008636	Specimen Disc, Assembly Specimen disc 20 mm
14041619275	Specimen disc, 25mm Specimen disc 25 mm
14037008587	Specimen disc, 30mm Specimen disc 30 mm
14037008637	Specimen disc, 40mm Specimen disc 40 mm
14041926491	Specimen disc, 55mm Specimen disc 55 mm
14041926750	Specimen stage Specimen disc 50 x 80 mm
14041638207	Transfer block Transfer block for specimen discs (LxWxH): 190 x 75 x 25 mm
14044326836	Heat extractor Heat extractor - mobile
14039818542	Thermal block Thermal block for removal of frozen specimens from specimen disc
14043626747	Miles adapter Adapter for Miles TissueTek® specimen discs
14044325949	90° prism 90 ° Prisma for direct specimen freezing on specimen head with clamping screw to facilitate specimen trimming.
14041145349	Plug adapter set EU-UK Plug adapter set EU-UK, for use with EG1120, EG1130, CM1100, CM1510 S,CM1520, CM1850/UV, CM1860/UV, CM3050 S, SP1600 220/230 V/50 Hz versions only.