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Overview of Biopsy Procedures and Experiential Data Analysis from a Comprehensive US Hospital Network Breast Center
This article provides an overview of biopsy procedures with an example of observations in a comprehensive health network biopsy suite.
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H&E Staining Overview: A Guide to Best Practices
For routine diagnosis, the use of Hematoxylin and Eosin (H&E) is by far preferred for viewing cellular and tissue structure detail by pathologists.
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Fixation and Fixatives - Popular Fixative Solutions
In this fourth part of the Fixation and Fixatives series, we look at some of the many popular and traditional fixative solutions that have been used in histology for the last 100 years. This part has an overview of proprietary solutions and...
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An Introduction to Decalcification
A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared.
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H&E Basics Part 4: Troubleshooting H&E
Though the H&E stain is a relatively simple stain to perform, there are a variety of artifacts that can interfere with a good stain. Artifacts can be attributed to a variety of causes.
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Special Stains – Which One, Why and How? Part I: Mucins and Glycogen
Mucins are a part of a complex group called carbohydrates. Mucins are mucopolysaccharides; they are important in cell growth as they help regulate the flow of nutrients between capillaries and cells and are known as “The Glue of Life”.
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Special Stains – Which One, How and Why? Part II: Connective Tissue
Special stains" are processes that generally employ a dye or chemical that has an affinity for the particular tissue component that is to be demonstrated. This article includes the processes and protocols necessary to demonstrate connective tissue.
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Special Stains – Which One, Why and How? Part III: Microorganisms – Bacteria and Fungi
Microorganisms are living organisms which include bacteria, fungi, protozoa and viruses. Bacteria, fungi and protozoa can be identified and classified with histochemical procedures and viruses are generally identified with immunohistochemical procedures.
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Special Stain Techniques for the Evaluation of Mucins
Special stains that are used for the evaluation of mucins, mucin-like molecules and other carbohydrate containing macromolecules remain in demand and are utilized frequently in the histology laboratory.
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The Basic Chemistry of Hematoxylin
This article is a review of the current understanding of the chemistry of aluminum-based hematoxylin stains.