Tutorials

Geoffrey Rolls, BAppSc, FAIMS

Cindy Sampias, JD CT(ASCP)HTL

For routine diagnosis, the use of Hematoxylin and Eosin (H&E) is by far preferred for viewing cellular and tissue structure detail by pathologists. We continue to use this simple and essential stain today, which has remained unchanged for well over a century.

Geoffrey Rolls, BAppSc, FAIMS

In this fourth part of the Fixation and Fixatives series, we look at some of the many popular and traditional fixative solutions that have been used in histology for the last 100 years. This part also has an overview of proprietary solutions and...

Geoffrey Rolls, BAppSc, FAIMS

A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared.

Cindy Sampias, JD CT(ASCP)HTL

Though the H&E stain is a relatively simple stain to perform, there are a variety of artifacts that can interfere with a good stain. Artifacts can be attributed to a variety of causes.

Carolyn Doan, HT (ASCP)

“Special stain” is a term used to refer to many alternative staining techniques that are used when the traditional H&E does not provide all the information the pathologist or researcher needs from a tissue slide.

Carolyn Doan, HT (ASCP)

"Special stain" is a term used to refer to many alternative staining techniques that are used when the traditional H&E does not provide all the information the pathologist or researcher needs from a tissue slide.

Carolyn Doan, HT (ASCP)

Microorganisms are living organisms which include bacteria, fungi, protozoa and viruses. Bacteria, fungi and protozoa can be identified and classified with histochemical procedures and viruses are generally identified with immunohistochemical procedures.

Russell Myers, Ph.D.

Special stains that are used for the evaluation of mucins, mucin-like molecules and other carbohydrate containing macromolecules remain in demand and are utilized frequently in the histology laboratory.

Russell Myers, Ph.D.

This article is a review of the current state of understanding of the chemistry of aluminum based hematoxylin stains.

Dr. Stephen Peters

This article describes a system used for embedding of tissues for the preparation of frozen sections. This novel system uses simple techniques and apparatus to accomplish face-down embedding in freezing-temperature steel wells.