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101 Steps to Better Histology - a Practical Guide to Good Histology Practice
This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors.
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An Introduction to Specimen Processing
"Tissue processing” describes the steps required to take animal or human tissue from fixation to the state where it is completely infiltrated with a suitable histological wax and can be embedded ready for section cutting on the microtome.
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An Introduction to Specimen Preparation
An introduction to the preparation of specimens for microscopy in the histopathology laboratory.
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Immunohistochemistry: An Overview + Steps to Better IHC Staining
Immunohistochemistry (IHC) is used in histology to detect the presence of specific protein markers that can assist with accurate tumor classification and diagnosis. This article provides an overview and the basic steps used to create an optimal IHC stain.
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Steps to Better Grossing
Get tips for better grossing in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.
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Steps to Better Specimen Collection + Transport
Get tips for better specimen collection and transport in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.
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Science of Tissue Processing
Tissue processing is a procedure of removing water from cells and replacing it with a medium which solidifies allowing thin sections to be cut on a microtome.
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Troubleshooting & Reprocessing Difficult Paraffin Blocks
From time to time, in all histopathology laboratories, paraffin blocks will be encountered that are difficult or even impossible to section.
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Science of H&E
H&E stain makes use of a combination of two dyes – hematoxylin and eosin. It is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen.
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Steps to Better ISH
Get tips for better ISH staining in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.