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BOND RX Fully Automated Research Stainer


Find Breakthroughs Faster with the Freedom to Discover

Speed up your next big discovery. Use the BOND RX to confidently complete IHC, ISH, FISH, CTC, multiplexing and other tests. Explore your ideas in a variety of ways via open reagents, open detection kits, and customizable protocols. The BOND RX provides an easy way for labs to fully automate tests and accelerate research programs.

  • Accelerate test programs through speed, efficiency, and consistent automation, reducing manual work so that researchers can spend less time at the bench and more time innovating.
  • Preserve morphology and integrity of precious tissue with the unique Covertile system that allows you to dispense reagents in a highly-controlled and more consistent manner. 91% of customers find the stain quality and reproducibility to be better than the competition.*
  • Commercialize your discovery with a clear path from research to clinical applications, and leverage greater access to innovative emerging technologies via partnerships.
Contact us to get a quote

For Research Use Only. Not for use in diagnostic procedures.
*Independent Studies


Product Features

Flexible Protocols Help Turn Theories Into Action

BOND RX lets researchers fully automate emerging technologies (including new molecular applications), customize all protocol segments and put theories into action.

  • Preparation - Customize your baking and dewax options.
  • Antigen Retrieval - Change Incubation time and temperature as desired.
  • ISH Probes – Leverage new probe application and removal options.
  • Staining – Choose your incubation times, temperatures, dispense types and more.
Flexible Protocols Help Turn Theories Into Action
Get Started Right Away with BOND RX

Get Started Right Away

BOND RX makes it easy to run the unique reagents you’re working with and quickly automate innovative tests. In fact, you can simply pipette directly into open containers to get your antibody or probe onto the BOND RX.

  • Create your own detection kits using simple and efficient research detection kits.
  • Fully automate staining with extremely small volumes of high value reagents using Titration containers.

Do More in Less Time with BOND RX

BOND RX helps research labs work more efficiently because it streamlines the test management process and makes it more flexible.

  • Save time by having antibodies, probes and detection systems accessible at any time.
  • Eliminate mundane tasks and minimize maintenance.
  • Reduce user strain with low-weight containers that are easy to handle.
  • View bulk reagent levels at a glance with a unique active lighting system.
Do More in Less Time
Be Confident In Results With Proprietary Covertile Technology

Be Confident In Results With Proprietary Covertile Technology

The BOND Covertile protects tissue morphology and enables gentle and consistent reagent application, unlike competitors who may not use a Covertile at all, or not for all steps of the process.

Commercialize Your Discovery

You gain tools to customize protocols and streamline the transition to clinical. The shared core technology provides you with a clear path from research to clinical.

Fully Transparent Operations

Researchers are in full control with BOND RX. It provides the insight you need to have a complete understanding of each test. You can view reagent application, monitor protocol progress, receive comprehensive reports and more.


Access the Latest Research Advances

Leica Biosystems partners with a range of Open Innovation (OI) partners. As a result, you have many flexible solutions for your research. All protocols and protocol templates developed in conjunction with our OI partners are available so you can access the latest research advances in the shortest time.

Multiplex Staining With No Intervention

Partner - Perkin Elmer

Free up researchers’ time by creating beautiful, seven-color immunofluorescence slides without any intervention. Up to 30 slides can be created in about 14 hours.

  • Visualize up to six tissue biomarkers at the same time plus a nuclear counterstain.
  • Use the best primary antibodies – regardless of species – with no crosstalk.
  • Optimize editable protocol with ease so it meets your specific needs.

Watch the Partnership Video - PerkinElmer Opal™

Multiplex Staining With No Intervention
A Fully Integrated Rna Ish Solution

A Fully Integrated RNA ISH Solution

Partner - ACD

Complete up to 30 slides in a single 11-hour run for single plex (and a 14-hour run for duplex) with high-throughput staining. This solution is:

  • Highly specific – It amplifies signals using double z probe strategy and simultaneous background suppression strategy to ensure target-specific binding.
  • Sensitive & Quantitative – It detects single RNA molecules at single cell resolution.
  • Morphological – It provides cell specific expression in information intact tissue architecture.
  • Universal – Use it for virtually any gene, species or tissue.

Watch the Partnership Video - ACD RNAscope

Further Metastasis And Residual Cancer Studies

Partner - Clearbridge

When combined with the BOND RX, Clearbridge CTC Enrichment Analysis lets researchers identify early, metastatic or residual cancers. It does so by staining enriched CTC cells from blood, rather than the physical tumor. You get:

  • Label-free CTC enrichment of heterogeneous populations of cancer cells.
  • Up to 10,000 times enrichment of CTCs that are wholly intact viable and assay ready.
  • Large blood volume enrichment from 7.5 mL of a blood sample.
CTC Analysis For Experimental Pathology Labs

CTC Analysis For Experimental Pathology Labs

Partner - Rarecyte

The AccuCyte-Cytefinder system integrates with the BOND RX and provides microscopic visualization of circulating tumor cells. It delivers single cell retrieval of target cells for downstream genomic analysis.

  • AccuCyte collects nucleated cells including circulating tumor cells from whole blood.
  • CyteSealer makes collection seamless by creating a barrier between the buffycoat and red blood cells.
  • CyteSpreader ensures the precise amount of nucleated cells is spread onto each slide every time.

Single Step Multiplexing, Fully Automated

Partner - Ultivue

Create up to 30, five color, single step multiplexing slides in only 4.5 hours on BOND RX whilst preserving tissue morphology and integrity. Spend less time perfecting difficult manual techniques and more time focusing on your next research breakthrough.

  • Survey and analyse cell phenotypes with spatial context within the tumor microenvironment though high performance tissue-based biomarker multiplex assays
  • Multiplex on a single slide, using a single antibody cocktail and incubation step, powered by DNA-barcoded antibodies with unique, complementary, fluorescent DNA barcodes
  • Preserve sample integrity for additional analyses without damaging tissue or cross talk
  • Image and analyse with Aperio VERSA and Aperio Cellular IF Algorithm
Single Step Multiplexing, Fully Automated


Product Specifications

Dimensions & Weight

Dimensions (W x H x D): 790 mm x 1378 mm x 806 mm
(31.1 x 54.25 x 31.73 in)
Weight (Dry): 238 kg (525 lbs)


Operating Voltage (older-model power supply; one fan in back cover): 103.4 V to 127.2 V (for nominal voltage 110 V to 120 V)
Power Consumption: 1200 VA
Operating Voltage (newer-model power supply; two fans in back cover): 90 V to 264 V (for nominal voltage 100 V to 240 V)
Mains Frequency: 50/60 Hz

Environmental (Unpacked Instruments)

Maximum Operating Temperature: 35°C (95°F)
Minimum Operating Temperature: 5°C (41°F)
Temperature Required to meet Staining Performance Requirements: 18–26°C (64–79°F)
Maximum Heating Energy Output: 1200W/1000W


Operating Humidity (Non-Condensing): 30 to 80% RH
Maximum Operating Altitude: 0 to 1600 m (5250 ft.) above sea level
Sound Pressure Level Output (at 1 m): < 85 dBA maximum
< 65 dBA normal operation


Slide Capacity: 30
Finished trays (10 slides) may be replaced continuously
Number of Reagent Containers: 36
Reagent Container Capacity: 7 mL and 30 mL
Titration Container Capacity: 6 mL
Bulk Reagent Container Capacity: 2 L or 5 L
Hazardous Waste Container Capacity: 5 L
Standard Waste Container Capacity: 2 x 5 L
Chemical Compatibility: All BOND Reagents
70% Alcohol Solution (for cleaning purposes)
Temperature Indication: Defaults (these can be changed by service representatives): Warm: 35°C, Hot: 80°C
Maximum permitted pressure for gas and liquid connections: 1.0 bar

Microscope Slides

Slide Width: 24.64–26.0 mm (0.97–1.02 in)
Slide Length: 74.9–76.0 mm (2.95–2.99 in)
Slide Thickness: 0.8–1.3 mm (0.03–0.05 in)
Label Area Width: 24.64–26.0 mm (0.97–1.02 in)
Label Area Length: 16.9–21.0 mm (0.67–0.83 in)
Material: Glass, ISO 8037/1

Transport and Storage (Packed Instruments)

Storage Temperature: -20 to 55 °C (-4 to 131 °F)
Storage Humidity (Non-Condensing): < 80% RH
Shipping Methods: Road and Air Freight Compatible


Novocastra HD Antibodies Deliver Higher Optimal Staining Rates Compared to Leading Vendors

Novocastra HD Antibodies Deliver Higher Optimal Staining Rates Compared to Leading Vendors

To deliver the performance you demand, our Novocastra HD line is independently evaluated by external quality assurance organization NordiQC. Head-to-head comparisons against other leading vendors show that our antibodies consistently deliver superior staining quality.

Exceptional Sensitivity for Exceptional Detection with Compact Polymer Technology

  • Lean efficiency – full automation reduces waste and process variation
  • Complete system – all aspects of the system optimized to work together to support your entire process
Code Name Show all details Configuration Use Datasheet MSDS Qty
DS9800 Bond Polymer Refine Detection Kit DS Info MSDS
DS9390 Bond Polymer Refine Red Detection Kit DS Info MSDS
DS9477 ChromoPlex 1 Dual Detection for BOND Kit DS Info MSDS

Research Detection Systems

Code Name Configuration Use Datasheet MSDS Qty
DS9777 Bond Research Detection System 2
DS9455 Bond Research Detection System
DS9263 Bond Intense R Detection System Kit DS Info MSDS
Code Name Configuration Datasheet MSDS Qty
S21.4611 BOND Universal Covertiles (pack of 160)
S21.4564 Bond Slide Labels and Printer Ribbon
S21.4604.B BOND Printer Ribbon & Labels Cxi (1Pack)
S21.4610.B BOND Printer Ribbon & Labels Cxi (6Pack)
S21.1003 Bond™ Reagent Tray
S21.0605 Bond™ Aspirating Probe
OP309700 Bond Open Containers 30ml
S21.1971 Bond™ Mixing Stations
OPT9719 Bond Titration Inserts 50x
OPT9049 Bond Titration Kit
OP79193 Bond Open Containers 7ml
CS9100 Bond Aspirating Probe Cleaning Kit Kit Info MSDS



BOND RTU Antibody and Protocol Data Files

These data files will enable the use of the latest protocol releases for BOND RX/RXm. Local licensing restrictions may apply to some products; please contact your local Leica Biosystems Sales or Support representative should you have any queries. Please ensure the correct data file is downloaded.

Important Notes

  • Do not apply this update to BOND-III or BOND-MAX Systems.
  • Please download and apply the correct update is for your region.
  • We recommend that all BOND systems be updated to the latest software version - RX v6.0
  • Do not apply this update to Systems running RX/RXm v4.0 software; this file is not applicable to software v4.0 Systems.
  • BXDv17 must be applied to Systems running RX/RXm software versions 5.2 and v6.0

New Protocols and Products Available in BXDv17:


  • Ultivue UltiMapper Assay


  • PA0094: Cytokeratin (AE1/AE3), 7 mL
  • PA0051: Muc-1 Glycoprotein (Ma695), 7 mL
  • PA0052: Muc-5AC Glycoprotein (CLH2), 7 mL
  • PA0053: Muc-6 Glycoprotein (CLH5), 7 mL
  • PA0831: Anaplastic Lymphoma Kinase (5A4), 7 mL.
  • PA0832: Programmed Death Ligand 1 (73-10), 7 mL
  • PA0050: Herpesvirus (Type 8) (13B10), 7 mL.


Partnership - PerkinElmer Opal™ Multiplex IHC Protocol

Partnership - ACD RNAscope: From Manual Research to Fully Automated Clinical Platform

Covertile System – Reagent Application Virtual Demo