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Aperio AT2 – High Volume, Digital Whole Slide Scanning

400 slide capacity brightfield scanner.
High throughput with small footprint.
2x3 slide option available.

We understand that your slides are more than just glass, they hold vital information for your work. With its compact size, high capacity autoloader and optimal image quality, the Aperio AT2 slide scanner has set new standards in digital pathology.

Aperio AT2 consistently delivers precise, whole slide images with an unparalleled, low rescan rate. Slides are available for remote viewing in less than a minute. With an easy-to-use Pathologist's cockpit, coupled with easy integration into laboratory information systems (LIS), the Aperio AT2 provides an ideal platform for research institutions.

The Aperio AT2 runs on the Microsoft Windows 10 operating system.


  • Z-stacking with up to 25 layers
  • Digitized slides are on the Aperio Digital Slide tray ready to use in less than 60 seconds
  • Easily load and capture images of an entire carousel in less than 8 hours
  • Adjustable tissue finder, if needed
  • Sustained high throughput rate of 50 slides/hr. @20x
  • Highly reliable autoloader with 400-slide capacity
  • Smaller file size and faster scanning with skip blank stripes technology
  • 98% first-time success rates with fewer rescans
  • Quiet, with minimal movement of slides in loader
  • Highly published and referenced whole slide scanner


Capacity2x3 or 400 slides (1" x 3")
Input Format1-inch x 3-inch (2.54 cm x 7.62 cm) microscope slides with 0.17 mm coverslip
2-inch x 3-inch (5.08cm x 7.62cm) microscope slides with 0.17 mm coverslip
DimensionsApproximately 23.5 inches (H) x 16 inches (W) x 25.5 inches (D)
59.7 cm (H) x 40.6 cm (W) x 64.7 cm (D)
WeightApprox. 129 lbs (58.5 kg)
Objective Lenses20x/0.75 NA Plan Apo (40x scanning with 2x optical mag changer)

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Complete digital pathology solutions

With an easy-to-use Pathologist’s cockpit, coupled with easy integration into the laboratory information system (LIS), the Aperio AT2 provides an ideal platform.

Improved automation

*98% of slides are captured first time in automatic mode, streamlining workflow and eliminating time spent on rescans. The LED light source and calibration controls ensure consistent, high quality scanning.

*Based on a Aperio AT2 scanning a 15x15mm area at 20x magnification.

Small footprint, high throughput

The streamlined design of the Aperio AT2 with compact carousel for up to 400 slides and low operating noise, means it takes up minimal bench space allowing busy, crowded labs to readily implement digital pathology.

Tissue Finder

The Aperio AT2 tissue finder is very robust but flexible when needed. The area of interest can be adjusted by the user to optimize tissue finding on difficult samples (such as fatty tissue) or to not detect ink or stain residue

Key downloads

 Specs (pdf)   Press Release

Educational Resources

Discover Digital Pathology

Four Tips for Digital Pathology Slide Scanning

Four Tips for Digital Pathology Slide Scanning

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Is a Digital Pathology Scanning & Hosting Service Right for You?

Is a Digital Pathology Scanning & Hosting Service Right for You?

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What is Digital Pathology?

What is Digital Pathology?

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Top Considerations When Buying a Digital Pathology Scanner

Top Considerations When Buying a Digital Pathology Scanner

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Capacity 2x3 or 400 2x3 or 5 8 or 200 4
Illumination Brightfield Brightfield Brightfield, 7-channel fluorescence & FISH Brightfield
Z stacking Yes Yes Yes Yes
Live View No No No Yes
Magnification 20x, 40x 20x, 40x 1.25x, 5x, 10x, 20x, 40x, 63x (oil or dry) 1.25x, 5x, 20x, 40x
Scan Method TDI line scan TDI line scan Tile Tile

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"We've used Aperio instruments to scan well over 100,000 slides and had great success with the images that were generated using them" Source: TechValidate. TVID: 445-F03-4C1

Imaging, Non Profit

For Research Use Only. Not for use in Diagnostic Procedures.

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