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Fundamentals of H&E Staining

The aim of this downloadable training resource is to help explain the fundamentals of H&E, or Routine Staining as it’s often termed.

Routine H&E staining plays a critical role in tissue-based diagnosis or research. By coloring otherwise transparent tissue sections, this stain allows highly trained pathologists and researchers to view, under a microscope, tissue morphology. This information is often sufficient to allow a disease diagnosis.

Learn more about:

  • What is “Routine Staining”
  • Histology routine workflow
  • Steps leading up to H&E staining

    • Proper techniques and common mistakes in

      • Processing
      • Microtomy

  • Science of H&E staining

    • Staining procedure methods
    • Staining methods
    • Staining dyes and ancillaries

  • Examples of staining protocols

    • Standard
    • Using xylene substitutes
    • For frozen sectioning

Download the Fundamentals of H&E Staining PDF now!

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