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Fundamentals of H&E Staining

Andrew Lisowski, M.S., HTL (ASCP)

The aim of this downloadable training resource is to help explain the fundamentals of H&E, or Routine Staining as it’s often termed.

Routine H&E staining plays a critical role in tissue-based diagnosis or research. By coloring otherwise transparent tissue sections, this stain allows highly trained pathologists and researchers to view, under a microscope, tissue morphology. This information is often sufficient to allow a disease diagnosis.

Learn more about:

  • What is “Routine Staining”
  • Histology routine workflow
  • Steps leading up to H&E staining
    • Proper techniques and common mistakes in
      • Processing
      • Microtomy
  • Science of H&E staining
    • Staining procedure methods
    • Staining methods
    • Staining dyes and ancillaries
  • Examples of staining protocols
    • Standard
    • Using xylene substitutes
    • For frozen sectioning

Download the Fundamentals of H&E Staining PDF now!

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About the presenter

Andrew Lisowski, M.S., HTL (ASCP)

Andrew Lisowski has almost 30 years of experience in histology and histotechnology. He attended veterinary school and earned his master’s degree in molecular biology. Andrew worked in histology, IHC and ISH labs, cell culture lab, performed in-vitro and in-vivo toxicology assays and was a member of a necropsy team. He worked for pharmaceutical companies, medical school and founded his own molecular and histology firms. Currently, Andrew is the Senior Technical Applications Manager at Leica Biosystems providing global technical support and training.

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