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Fundamentals of H&E Staining

Overview

H&E staining is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen. H&E is a fast and relatively inexpensive method of assessing tissue morphology.

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Learning Objectives

  • What is “Routine Staining”
  • Histology routine workflow
  • Steps leading up to H&E staining

    • Proper techniques and common mistakes in

      • Processing
      • Microtomy

  • Science of H&E staining

    • Staining procedure methods
    • Staining methods
    • Staining dyes and ancillaries

  • Examples of staining protocols

    • Standard
    • Using xylene substitutes
    • For frozen sectioning

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