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Effects of decalcification on In Situ Hybridization Stains and Immunohistochemical Stains


Publications describing the effects of decalcification on immunohistochemistry (IHC) staining have been published intermittently with limited new data since the 1990s.  At the same time IHC stains have become an ever more important and dependent tool in anatomic pathology for diagnosis, prognosis, and treatment purposes. Current instrumentation, in addition to being fully automated, typically use polymer based detection systems, in place of avidin-biotin complex based detection, which was predominate when most of the previous studies were performed. This presentation will review performance of ISH and IHC markers decalcification reagents, and antibodies with current detection system of polymer based detection.   Also, a review of current IHC validation recommendation concerning decalcified tissue will be discussed.

Learning Objectives:

  1. Characterize performance of ISH markers; kappa and lambda, with current decalcification reagents will be reviewed.
  2. Characterize performance of 33 different IHC markers with current decalcification reagents will be reviewed.
  3. Review current IHC validation recommendations concerning decalcified tissue currently in the USA.

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