Skip to main content

Effects of decalcification on In Situ Hybridization Stains and Immunohistochemical Stains

Publications describing the effects of decalcification on immunohistochemistry (IHC) staining have been published intermittently with limited new data since the 1990s.  At the same time IHC stains have become an ever more important and dependent tool in anatomic pathology for diagnosis, prognosis, and treatment purposes. Current instrumentation, in addition to being fully automated, typically use polymer based detection systems, in place of avidin-biotin complex based detection, which was predominate when most of the previous studies were performed. This presentation will review performance of ISH and IHC markers decalcification reagents, and antibodies with current detection system of polymer based detection.   Also, a review of current IHC validation recommendation concerning decalcified tissue will be discussed.

If you have viewed this educational webinar, training or tutorial on Knowledge Pathway and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript.
Get Knowledge Pathway updates delivered directly to your inbox.

Learning Objectives

  1. Characterize performance of ISH markers; kappa and lambda, with current decalcification reagents will be reviewed.
  2. Characterize performance of 33 different IHC markers with current decalcification reagents will be reviewed.
  3. Review current IHC validation recommendations concerning decalcified tissue currently in the USA.

Leica Biosystems Knowledge Pathway content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only.

For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted.

Copyright © 2022 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.

About the presenter

Dr. Philip Ferguson is in a private practice located in Arkansas. He has a diverse training background that includes an undergraduate degree in mechanical engineering, post-graduate training in internal medicine, and board certifications in anatomic pathology, clinical pathology, and hematopathology. His professional interests center around educational resource development for pathologists and trainees, and use of immunohisochemistry and molecular diagnostics in surgical pathology. Dr. Ferguson launched one of the first widespread internet educational tools for pathology residents to prepare for their board examination, PathMD (, which is now the Leica tool PathLead. Dr. Ferguson also serves as a state commissioner for the College of American Pathologists' Accreditation and Laboratory Improvement Program and is also member of the Global Advisory Board for Leica Biosystems. Dr. Ferguson has published several publication of late including ‘The Effect of Decalcification Solutions on Kappa and Lambda In-Situ Hybridization,’ as well as ‘Effects of Decalcification on Immunohistochemistry Comparing: Immunocal®, Formical2000®, and EDTA Stat®.’

Want us to feature your writing?
We are looking for more great writers to feature here. Send us a submission and we’ll be in touch!
Like what you see?
Get more Knowledge Pathway content delivered directly to your inbox. Unsubscribe at any time.