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101 Steps to Better Histology - a Practical Guide to Good Histology Practice

From patient to pathologist, preparing tissue specimens for histological examination requires care, skill and sound procedures. This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors. Each aspect of the histology process is covered:

  • Specimen collection
  • Grossing
  • Tissue processing
  • Paraffin embedding
  • Sectioning and microtomes
  • Routine staining (H&E)
  • Special stains
  • IHC staining (immunohistochemistry)
  • ISH staining (in situ hybridization).

Each of the 101 steps provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

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References

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