HALO® | Quantitative Image Analysis for Pathology

Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.

Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.

Simultaneously analyze up to three chromogenic and/or silver-labelled DNA or RNA ISH probes on a cell-by-cell basis, measuring spot numbers and area per cell and compartment, and calculated H-scores for each probe.

Simultaneously analyze a nuclear stain and up to four IHC biomarkers or ISH probes on a cell-by-cell basis across brightfield images.​

Simultaneously analyze an unlimited number of fluorescent nucleic acid probes on a cell-by-cell basis, measuring spot numbers and area per cell and compartment, and calculated H-scores for each probe.

Use the HALO® FISH-IF module and reagents from Molecular Instruments or ACD, a Bio-techne brand, to simultaneously analyze an unlimited number of fluorescently-labeled DNA/RNA ISH probes and immunofluorescent protein biomarkers on a cell-by-cell basis.

The TMA Add-on provides a productivity-enhancing workflow for tissue microarray analysis by enabling automated, high-throughput segmentation and batch analysis of whole slide TMA images.

Analyze serial tissue sections stained with different markers or a single tissue section which has been stained, stripped, and re-stained for multiple markers.

Deconvolve up to five colors in brightfield and measure positive area and average optical density for each stain and stain colocalization (where applicable).

Measure the positive area, average intensity and dye colocalization (where applicable) of an unlimited number of fluorescent dyes.

Simultaneously analyze up to five chromogenic stains and measure object density, area, diameter, and optical density, as well as colocalizations, if applicable.

Simultaneously analyze an unlimited number of fluorescent dyes and measure object density, area, diameter, and intensity, as well as colocalizations, if applicable.

Quantify the area, diameter, perimeter, and number of white spaces per region of interest in brightfield images. Ideally suited for analysis of lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung.

Quantify area, diameter, and perimeter of muscle fibers stained with laminin or other fiber membrane stains.

Quantify fiber or membrane positivity for an unlimited number of fluorescent dyes along with fiber diameter, perimeter, and area.

Quantify microglial activation based on length and thickness of microglial processes.

Quantify microglial activation in fluorescence based on detection of microglia, soma, and processes, by counting branch points, and by determining area, length, and thickness of processes.

Quantify branch ends, area, length, and more for branched structures such as retinal vessels and cortical neurons in brightfield images.

Quantify branch ends, area, length, and more for branched structures such as retinal vessels and cortical neurons in fluorescent images.

Quantify axons in nerve cross sections and calculate area, inner and outer diameter, myelin area, and G-ratio.

Count and measure pancreatic islets in brightfield images and quantify the islet area and cells positive for up to two islet-specific stains.

Count and measure pancreatic islets in fluorescent images and quantify the islet area and cells positive for up to three islet-specific dyes.

Process multiple images in parallel to maximize image analysis throughput and minimize analysis job turnaround times.