Slide 1 Welcome! Slide 2 Intro slide Slide 3 It's nice to meet you! Welcome to the Histology Hacks in Cryosectioning webinar. I am Dr. Marla Rivera-Oliver, and I am very excited to share today some tips and tricks I've learned during my ten years of experience working on basic and translational research. I am based in the Dallas, Texas, area of the United States, and in my current role, I support customers from North Texas and Oklahoma. I completed a bachelor's in biology from the Interamerican University of Puerto Rico and a doctoral degree in Neurobiology from the University of Puerto Rico. I specialized in the spinal cord injury field and honed my skills in Cryosectioning, imaging, and in vivo electrophysiology. Then, I moved into a post-doctoral position at the Center for D...

In the webinar Cryosectioning 101 for Research, John Stock provided a comprehensive guide on achieving better cryosectioning outcomes. John went on to answer customer questions at the end of the session, that have been compiled for your viewing here.

Dieser Artikel beschreibt ein System zur Einbettung von Geweben, die für die Gefrierschnitterstellung vorbereitet werden. Das System ist einfach zu erlernen und bietet zahlreiche Vorteile gegenüber herkömmlichen Methoden, so etwa hinsichtlich Geschwindigkeit, Präzision und Planbarkeit sowie reduziertem Gewebeverlust.

The Brain Punch Tissue Set is used for the Palkovits Punch technique, which allows extraction of minute amounts of brain tissue from exact locations in the brain. A hypodermic tube with a sharp rim is pressed onto a frozen section on a glass slide. The tissue under the hole is freed and pulls away with the punch. It can then be expelled from the punch with the spring loaded expeller by pressing the top of the punch. The Brain Punch Tissue Set co...

The CryoJane Tape-Transfer System for Cryosectioning makes it easy to produce high-quality frozen sections of difficult tissues. The CryoJane uses adhesive coated slides and adhesive tapes to capture sections instead of using an anti-roll plate or brush. The Leica CryoJane is adaptable to virtually all cryostats on the market. The system achieves frozen sections of paraffin-quality as thin as two microns. The sections are wrinkle-free, uncompres...

A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy...

Andrew will explain how glass is made and how we change its surface, so it is not inert.He will also review the common factors contributing to specimen loss and describe the benefits and dangers of the adhesives currently used. These modifications dictate the...

In this session, we will briefly review the basics of molecular biology, examine critical factors which affect the quality of nucleic acids in the tissues and cells which are submitted for downstream molecular diagnostics, and briefly introduce some...

In surgical pathology more than ever, the laboratories are expected to do more with less. Taking on more specimens with less technical staff, or getting more stains out of tiny biopsies or fine needle aspirations have become the norm. The College of American...