Histology Sample Preparation


Histology sample preparation prepares tissue specimens for sectioning, staining and diagnosis. The standard paraffin process (tissue processing) moves specimens through a series of steps so the soft tissue is supported in a medium that allows sectioning. The standard steps are: Fixation that preserves the tissue, Processing that dehydrates, clears and infiltrates the tissue with paraffin wax, Embedding that allows orientation of the specimen in a “block” that can be sectioned and is easy to store and handle, and Sectioning using a microtome to produce very thin sections that are placed on a microscope slide ready for staining. Frozen sectioning is an alternative preparation technique that quickly freezes tissue to preserve it and provide sufficient hardness so it can be sectioned immediately using a cryostat. This technique is often used during surgery where the surgeon needs to locate a tumor margin to ensure it has all been removed.



Article

An Introduction to Decalcification

Author:
23. Oct 2012
A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared. Read article

Useful Links

Human Histology & Introductory Pathology

histology.leeds.ac.uk/what-is-histology/
Basic introduction to histology

www.pathologyoutlines.com/
Comprehensive textbook of surgical and clinical pathology

pathmd.com
Educational tools for pathology residents and physicians

www.path.uiowa.edu/virtualslidebox/
Virtuel Slidebox for histology and histopathology

teaching.anhb.uwa.edu.au/mb140/
Blue histology - broad coverage of histology

library.med.utah.edu/
Health science library

Histotechnology

stainsfile.info/StainsFile/jindex.html
Useful information about dyes, stains and staining

www.ihcworld.com/index.htm
Information Source for immunohistochemistry

www.bristol.ac.uk/vetpath/cpl/histmeth.htm
Useful information on staining techniques

Organizations and Discussion Groups

www.histonet.org/
Histonet - listserver for the histology profession

www.nsh.org/
The National Society for Histotechnology

www.hgv.org.au/
The Histology Group of Victoria

www.hgq.org.au/
Histotechnology Group of Queensland

www.histonsw.org.au/
Histotechnology Group of NSW

Quality Assessment Programs

www.ukneqas.org.uk
United Kingdom National External Quality Assessment Service

www.nordiqc.org/
Nordic Immunohistochemical Quality Group

www.rcpaqap.com.au/
The Royal College of Pathologists of Australia

www.nsh.org/content/all-about-histoqip
HistoQIP quality assurance program 

Search Engines

www.histosearch.com/
Search engine for histology information

www.antibodybeyond.com/
Comprehensive information source about antibodies and IHC

www.gopubmed.com
Search interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines
List of academic databases and search engines

scholar.google.com
Beta of Google's search engine for scientific abstracts

Journals

jcp.bmj.com/
Journal of Clinical Pathology

www.pathologyinpractice.com/
Pathology in Practice - practical solutions, news, case studies

www.doaj.org/
Directory of open access journals

www.sciencemag.org/
Science

www.nature.com/
Nature

www.plosone.org/home.action
International, peer-reviewed, open-access online publication

Useful Text Books

Human Histology & Introductory Pathology

Young B, O’Dowd G, Stewart W, (2010): Wheater's Basic Pathology: A Text, Atlas and Review of Histopathology (5th ed.)

Kerr JB. (2010): Functional Histology (2nd ed.)

Mescher AL. (2009): Jun queira's Basic Histology ( 12th ed.)

Eroschenko VP. (2007): diFiore's Atlas of Histology with Functional Correlations (11th ed.)

Young B, Lowe JS, Stevens A, Heath JW (2006): Wheater's Functional Histology (5th ed.)

Histotechnology

Stephen R. Peters (2009): A Practical Guide to Frozen Section Technique. Springer-Verlag.

Carson F, Hladik C. (2009): Histotechnology: A Self-Instructional Text. (3rd ed.)

Kiernan J. (2008): Histological and Histochemical Methods: Theory and Practice. (4th ed.)

Bancroft JD, Gamble M. (2007). Theory and Practice of Histological Techniques. (6 th ed.) online publication

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