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Sample Preparation

Some of the critical steps in tissue sample preparation include fixation, grossing, embedding, and sectioning. Visit this section for guidance through the entire process.

101 Steps to Better Histology - a Practical Guide to Good Histology Practice

Geoffrey Rolls, BAppSc, FAIMS

This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors.

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The Art of Frozen Tissue Sectioning

Dr. Stephen Peters, MD

This article describes a system for embedding of tissues for the preparation of frozen sections. The system is easy to learn and offers many advantages over conventional methods, including speed, high precision and predictability, and reduced tissue wastage.

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Introduction to Microtomy: Preparing & Sectioning Paraffin Embedded Tissue

Geoffrey Rolls, BAppSc, FAIMS

Get tips for better microtomy, flotation & section drying in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

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An Introduction to Decalcification

Geoffrey Rolls, BAppSc, FAIMS

A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared.

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Processing Fatty Specimens

Andrew Lisowski, M.S., HTL (ASCP)
Adam Walter, CSSMBB, CLA, BS

One of the most critical steps in histology is fixation, especially when it comes to fatty tissue.

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An Introduction to Specimen Preparation

Geoffrey Rolls, BAppSc, FAIMS

An introduction to the preparation of specimens for microscopy in the histopathology laboratory.

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