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Introduction to Immunofluorescence


A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy and staining. Direct and indirect IF procedures will be discussed in detail. Technical comments of the many steps required for this technology will be given to allow the technologist a better understanding of the detail of attention required for this technology. Photomicrographs of finished slides will conclude this presentation.

Learning Objectives:

  1. Proper specimen handling.
  2. The importance of attention to detail.
  3. Reagents and supplies required for IF staining.
  4. Antibody staining patterns.

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