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With the advent of the molecular techniques in the past ten years, there has been a steady increase in the utility of the diagnostic and predictive biomarkers in human tumors. In this educational exercise we will first examine what is the nature of these markers and how they were discovered. We will then systematically review their current practical application in diagnostic immunohistochemistry by illustrating a number of case examples. Our discussion will be limited to the “diagnostic” markers as the field of “predictive” biomarkers is rather vast and requires a separate session. The presentation will conclude by summarizing the advantages and limitations of the application of genomically-derived antibodies in diagnostic immunohistochemistry.
- The origin of genomically-derived protein markers and the methods of their discovery.
- Their practical application to diagnostic tumor immunohistochemistry.
- The advantages and potential limitations of their utilization in histopathology.
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