101 Steps to Better Histology - a Practical Guide to Good Histology Practice

16. June 2008

From patient to pathologist, preparing tissue specimens for histological examination requires care, skill and sound procedures. This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors. Each aspect of the histology process is covered:

  • Specimen collection
  • Grossing
  • Tissue processing
  • Paraffin embedding
  • Sectioning and microtomes
  • Routine staining (H&E)
  • Special stains
  • IHC staining (immunohistochemistry)
  • ISH staining (in situ hybridization).

Each of the 101 steps provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

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Geoffrey Rolls

Geoffrey Rolls, Histology Consultant, Leica Biosystems, Melbourne, Australia, has extensive experience practicing and teaching histology and histotechnology including 30 years as Senior Lecturer in the Department of Laboratory Medicine at RMIT University in Melbourne, Australia. He holds a science degree and a professional Fellowship of the Australian Institute of Medical Scientists. He has a broad interest in histotechnology, particularly in the areas of tissue processing and microtomy and in general histology education. For the last ten years he has acted as a consultant to Leica Biosystems.

Geoffrey.Rolls@leicabiosystems.com

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