From patient to pathologist, preparing tissue specimens for histological examination requires care, skill and sound procedures. This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors. Each aspect of the histology process is covered:
- Specimen collection
- Tissue processing
- Paraffin embedding
- Sectioning and microtomes
- Routine staining (H&E)
- Special stains
- IHC staining (immunohistochemistry)
- ISH staining (in situ hybridization).
Each of the 101 steps provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.
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