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Decalcification: What You Need To Know


The content of this webinar will encompass detailed technical descriptions of novel and state of the art methods to decalcify tissue samples that will be embedded in paraffin or for frozen sectioning for molecular and nucleic acid analysis for basic research purposes. Procedures to be discussed include EDTA, Formic Acid, and rapid decalcifying, with discussion of key technical considerations and basic chemistry in each case. Troubleshooting and discussion of approaches to surface decalcify already embedded and cut tissues will also be discussed.

Learning Objectives:

  1. Review the various decalcification methods.
  2. Identify the appropriate decalcification method for your sample.
  3. Define the appropriate time needed for decalcification.

Sarah Mack has over a decade of experience as a histotechnician, with a main focus on preparation, processing and analysis of both calcified and soft tissues of the musculoskeletal system. In addition to providing consulting, advice and assistance to clients of the HBMI Core, Sarah serves as its Manager, supervising all histology-based projects within the Center of Musculoskeletal Research. Sarah supervises the generation of musculoskeletal tissue biorepositories, and oversees the creation and maintenance of reagent stocks and standard protocols. She provides hands-on assistance in the preparation of histological tissues, including decalcification, automated paraffin processing, orientation before embedding and sectioning of paraffin and frozen tissue.

Sarah currently serves as Chair of the Hard Tissue Committee for the National Society of Histotechnology and as President of the New York State Histotechnological Society. She has been an active member in both societies for more than 15 years, providing information and knowledge to histologists that work in both scientific and clinical lab settings.

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