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From Diagnosis to Treatment - Part 3 of 4, Diagnosis of Breast Cancer with H&E and Immunohistochemistry Stains


In the case of Breast Cancer, the patient’s cancer is usually detected by a mammogram or palpitation of an experienced Physician. However, these first indications that something is wrong must be confirmed by a more scientific approach. Usually what follows is an actual biopsy; a sample of the tissue in question is removed from the person. Once a tumor is confirmed from the biopsy, then a more radical surgical approach may be necessary or other treatments are performed. Part 3 of the series addresses the importance of proper H&E/ICH sections and staining for diagnosis of breast tumors.  A view of optimization and controls for prognostic markers will be examined, and brief overview of IHC diagnosis and treatment will presented.

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Learning Objectives

Discuss the importance of good H&E /IHC sections, and differences in the pathologist’s evaluation of core versus whole-section specimens.

  1. Understand the differential diagnosis of types of breast tumors.
  2. To view a good optimization of the antibody as an essential part of laboratory practice.
  3. Examine a variety of controls for prognostic (graded) IHC markers such as ER/PR/HER2.
  4. A brief overview of the next steps after IHC diagnosis and treatment. 

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