Developing Antibodies for Immunohistochemistry for Diagnosis in Formalin Fixed, Paraffin Embedded Tissues
The objectives of the presentation is to give an overview of monoclonal antibody production, specifically to give antibodies of good technical quality for diagnostic immunohistochemistry on formalin fixed paraffin embedded material. Methods of clone selection, antibody characterization and protocol optimization will be discussed.
- Review of principles of immunohistochemistry
- Description of how monoclonal antibodies are made and how clones are selected
- Overview of verification and validation testing performed on selected clones
- Description of how product claims are generated