Geoffrey Rolls

BAppSc, FAIMS

Geoffrey Rolls is a Histology Consultant with decades of experience in the field. He is a former Senior Lecturer in histopathology in the Department of Laboratory Medicine, RMIT University in Melbourne, Australia.

Published Pieces by Geoffrey Rolls

Artifacts in Histological and Cytological Preparations

Download this publication to learn more about the various artifacts which may be encountered in histopathology.

Introduction à la préparation des échantillons

"Tissue processing” describes the steps required to take animal or human tissue from fixation to the state where it is completely infiltrated with a suitable histological wax and can be embedded ready for section cutting on the microtome.

Vue d’ensemble de la coloration H&E : guide des bonnes pratiques

For routine diagnosis, the use of Hematoxylin and Eosin (H&E) is by far preferred for viewing cellular and tissue structure detail by pathologists.

L’immunohistochimie : un aperçu général + les étapes indispensables à une meilleure coloration IHC

Immunohistochemistry (IHC) is used in histology to detect the presence of specific protein markers that can assist with accurate tumor classification and diagnosis. This article provides an overview and the basic steps used to create an optimal IHC stain.

Process of Fixation and the Nature of Fixatives

Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue...

Steps to Better ISH

Get tips for better ISH staining in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

Introduction to Microtomy: Preparing & Sectioning Paraffin Embedded Tissue

Get tips for better microtomy, flotation & section drying in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

Steps to Better Grossing

Get tips for better grossing in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

Steps to Better Specimen Collection and Transport

Get tips for better specimen collection and transport in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

An Introduction to Decalcification

A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared.