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Tips & Tricks to Multiplexing: How to Choose Chromogen Colors for Multiplex and Detection Systems for Multiplex Assays

Rhian Evans, Ph.D., Scientist

Multiplexing is an important tool for simultaneous detection of multiple markers within a single tissue section.  

Watch our 2 short videos below to learn more about multiplexing and download the complementary infographics to keep the points on hand when you need to revisit the information!

How to choose chromogen colors for multiplex

 If you have opted for chromogenic multiplex staining, there are additional points to consider: the choice of colors and the order of color application.  While this video won’t tell you exactly what, when, and where to use different chromogens, it will provide you with some tips to consider.

Check out the infographic below and learn with Larry from Leica Biosystems about the tips to consider for chromogenic multiplex staining into your tissue-based research.

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Detection systems for multiplex assays  

There are many decisions that must be made when adopting multiplex staining, such as choosing chromogenic or immunofluorescent multiplex assays, as well as the order and colors of chromogens and fluorophores. However, the decisions don’t stop here as there are different detection systems available for multiplex assays that can increase sensitivity and enhance the signal-to-noise ratio when detecting markers of interest. We will cover a few of these examples in this video.

Unsure of what detection systems are available for multiplex assays? Check out the infographic below that showcases some examples of detection systems available for multiplex assays that can increase sensitivity and enhance the signal-to-noise ratio when detecting markers of interest.

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This article was created for Research Link, Leica Biosystems life science newsletter. Like what you read?

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About the presenter

Rhian Evans, Ph.D., Scientist

Rhian is a Scientist from Swansea University in Medical and Healthcare Studies and was featured in several collaborative publications. Rhian’s research-based background focused on tissue-based pathology in Multiple Sclerosis, primarily using immunohistochemical analysis and in vitro molecular techniques. She spent a short period conducting routine PCR testing for COVID-19 at the end of 2020.