Skip to main content

Design, Optimization, and Validation of Multiplex Immunofluorescence Panels

Michael J Surace
Michael J Surace Scientist II, AstraZeneca

In this webinar, we will cover biological and technical considerations when designing a panel, a linear process for developing, testing, and optimizing a panel, and an approach for technical validation of multiplex panels which detects and addresses known risks.

Multiplex immunofluorescence (mIF) combines the spatial information from immunohistochemistry ( IHC ) with multimarker phenotypes. Recent advances in mIF technology have made it possible for researchers to develop novel custom panels, but additional considerations must be taken into account in order to produce a panel which performs at least as well as IHC on a marker-by-marker basis, and avoids any undesirable interactions between detection of the targets and neighboring visible light spectra.

Learning Objectives

  • Attendees will learn about key factors to consider when designing a new mIF panel
  • We will walk through an efficient process for developing and optimizing mIF panels using TSA-linked fluorophores and iterative staining
  • Attendees will become familiar with the best use cases and advantages of most common mIF staining and imaging technologies
  • Attendees will come away from the webinar with an adaptable scheme for testing and validating a novel mIF panel

About the presenter

Michael J Surace
Michael J Surace , Scientist II, AstraZeneca

Michael Surace is a scientist at AstraZeneca, where he is responsible for the design, optimization, and validation of multiplex IF panels, as well as imaging and image analysis approaches specifically for the discovery of complex predictive and prognostic biomarkers for immune oncology.

Related Content

El contenido de Leica Biosystems Knowledge Pathway está sujeto a las condiciones de uso del sitio web de Leica Biosystems, disponibles en: Aviso legal.. El contenido, incluidos los webinars o seminarios web, los recursos de formación y los materiales relacionados, está destinado a proporcionar información general sobre temas concretos de interés para los profesionales de la salud y no está destinado a ser, ni debe interpretarse como asesoramiento médico, normativo o jurídico. Los puntos de vista y opiniones expresados en cualquier contenido de terceros reflejan los puntos de vista y opiniones personales de los ponentes/autores y no representan ni reflejan necesariamente los puntos de vista ni opiniones de Leica Biosystems, sus empleados o sus agentes. Cualquier enlace incluido en el contenido que proporcione acceso a recursos o contenido de terceros se proporciona únicamente por comodidad.

Para el uso de cualquier producto, debe consultarse la documentación correspondiente del producto, incluidas las guías de información, los prospectos y los manuales de funcionamiento.

Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.