New approach to preparing thin whole-body sections for histochemistry, immunohistochemistry, and light microscopical autoradiography.
(Tadafumi Kawamoto, Radioactive-Isotope Research Laboratories, Tsurumi University, School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-Ku, Yokohama, Japan)
This study presents a method for preparing fresh-frozen sections that can be used for histological, histochemical and immunohistochemical studies at light microscopical level with entire laboratory animals.
To achieve this, an adhesive film to support the frozen cut section was specially prepared. In addition, the cryomicrotome (CM 3500) and cryostat were modified. An entire child rat and a pregnant mouse were frozen in cooled hexane (-94°C).
The frozen animal was embedded in a 5% CMC gel. The animal and gel were completely frozen. The frozen CMC block was trimmed with a disposable tungsten carbide blade at approximately –23°C.
The exposed tissue surface was covered with an adhesive film and cut into 2-5 micro-meter thick sections.