Automated Multiplex Immunofluorescence
Cancer research continues to push the boundaries with new advancements in tissue analysis and biomarker detection. Now, more than ever, there is significant emphasis on understanding the underlying interaction between the immune system and the tumor microenvironment. Multiplexing immunofluorescence (mIF) has greatly increased our understanding of solid tumor biology and immunology, including tumor-infiltrating lymphocytes and cancer-induced architectural alterations, and aided in novel immunology discoveries. In this webinar, we will discuss how Akoya Biosciences Phenoptics assays support quantitative mIF to overcome the limitations imposed by conventional IHC methodologies. We will also discuss how our Opal assay kits and reagents can be integrated with the Leica BOND RX to automate your staining workflow to support consistent results for high-throughput studies.
Learning Objectives
- Understand the limitations of conventional IHC , and the benefits of transitioning to multiplex immunofluorescence
- Learn how to design, optimize and analyze your multiplex assay panel
- Understand how the BOND RX enables innovations like the Opal assay
About the presenters
Bethany Remeniuk is a Research Scientist with extensive working knowledge and diverse skillsets in the fields of cancer biology, medical pharmacology, and neurobiology. Accomplished in the development, design, and execution of both clinical and preclinical studies with successful history of publications. Able to effectively collaborate across different research specialties, both in academia and industry, to achieve work objectives
Traci DeGeer is the Director, Advanced Staining Innovation, Leica Biosystems. In this capacity she helps access new technologies for the Life Science research business, manages relationships with partners, works with legal partners to put agreements in place and liaises with Business Units to meet partner/customer needs as technologies are being developed. Traci holds a Bachelor of Science, in Biology, an HT, HTL, and QIHC for the anatomic pathology lab and recently graduated the HBx core program. Traci also holds a patent in small molecule detection for PDL-1 and has spoken at over one hundred state, regional and global symposia on various topics. Traci also sits on the ASCP Board of Certification (HT, HTL and QIHC Exam) and is the current Education Chair for the National Society of Histotechnology.
Related Content
Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only.
For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted.
Copyright © 2025 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.