In the webinar Cryosectioning 101 for Research, John Stock provided a comprehensive guide on achieving better cryosectioning outcomes. John went on to answer customer questions at the end of the session, that have been compiled for your viewing here.

The CryoJane Tape-Transfer System for Cryosectioning makes it easy to produce high-quality frozen sections of difficult tissues. The CryoJane uses adhesive coated slides and adhesive tapes to capture sections instead of using an anti-roll plate or brush. The Leica CryoJane is adaptable to virtually all cryostats on the market. The system achieves frozen sections of paraffin-quality as thin as two microns. The sections are wrinkle-free, uncompress...

Slide 1 Welcome! Slide 2 Intro slide Slide 3 It's nice to meet you! Welcome to the Histology Hacks in Cryosectioning webinar. I am Dr. Marla Rivera-Oliver, and I am very excited to share today some tips and tricks I've learned during my ten years of experience working on basic and translational research. I am based in the Dallas, Texas, area of the United States, and in my current role, I support customers from North Texas and Oklahoma. I completed a bachelor's in biology from the Interamerican University of Puerto Rico and a doctoral degree in Neurobiology from the University of Puerto Rico. I specialized in the spinal cord injury field and honed my skills in Cryosectioning, imaging, and in vivo electrophysiology. Then, I moved into a post-doctoral position at the Center for D...

This article describes a system for embedding of tissues for the preparation of frozen sections. The system is easy to learn and offers many advantages over conventional methods, including speed, high precision and predictability, and reduced tissue wastage.

A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy...

In recent years, there has been an increase of pathogens findings in tissues. This presentation will discuss some of the reasons for the increase of these pathogens in the past few years. The presentation will also will give an overview of why some of these...

In surgical pathology more than ever, the laboratories are expected to do more with less. Taking on more specimens with less technical staff, or getting more stains out of tiny biopsies or fine needle aspirations have become the norm. The College of American...

In this session, we will briefly review the basics of molecular biology, examine critical factors which affect the quality of nucleic acids in the tissues and cells which are submitted for downstream molecular diagnostics, and briefly introduce some...