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Tissue Processing

Tissue processing consists of dehydration, clearing, and infiltration. Here, experts share their recommendations for properly executing these steps.

An Introduction to Specimen Processing

Geoffrey Rolls, BAppSc, FAIMS

"Tissue processing” describes the steps required to take animal or human tissue from fixation to the state where it is completely infiltrated with a suitable histological wax and can be embedded ready for section cutting on the microtome.

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Tips and Tricks to Better Histology: Influencing Tissue Processing and Preparation

Fiona Tarbet, B.A.Sc, Leica Biosystems

In this webinar, Fiona Tarbet will investigate some of the effects of poor technique on section and stain quality and identify ways of producing better results.

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Troubleshooting & Reprocessing Difficult Paraffin Blocks

Geoffrey Rolls, BAppSc, FAIMS

From time to time, in all histopathology laboratories, paraffin blocks will be encountered that are difficult or even impossible to section.

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Processing Fatty Specimens

Andrew Lisowski, M.S., HTL (ASCP)
Adam Walter, CSSMBB, CLA, BS

One of the most critical steps in histology is fixation, especially when it comes to fatty tissue.

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Introduction to Immunofluorescence

James Burchette, HT (ASCP)

A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy...

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Artifacts in Histological and Cytological Preparations

Geoffrey Rolls, BAppSc, FAIMS
Neville J Farmer, DipMLT, FAIMS
John B Hall, MSc, FAIMS

Download this publication to learn more about the various artifacts which may be encountered in histopathology.

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Steps to Better ISH

Geoffrey Rolls, BAppSc, FAIMS

Get tips for better ISH staining in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

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Effects of Fixation and Tissue Processing on Immunocytochemistry

Effects of Fixation and Tissue Processing on Immunocytochemistry

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60 Minutes: 20 Histology Tips

Clifford Chapman, HTL(ASCP), QIHC (ASCP)

When was the last time that your pathologist brought you a slide of decalcified bone, and said it was the best she ever saw? Ever wonder why your PAS stain is not staining the basement membrane the way it should? These questions and 18 others will be discussed...

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