Skip to main content

The Power of Spatially Resolved Staining

Bradley Spencer-Dene Subject Matter Expert, Next Gen Histology at GSK
Elena Miranda, PhD Director, Non-Clinical Histology at GSK

Stay up-to-date with the latest research studies in spatially resolved staining techniques.

Chromogenic Co-detection of Protein and mRNA Targets in Tissues using the BOND RX Fully Automated Research Stainer

Bradley Spencer-Dene
Subject Matter Expert, Next Gen Histology at GSK

Co-detection of more than one target in Formalin-Fixed Paraffin Embedded samples is an extremely useful application and one that is increasingly required in modern histopathology labs. In order to detect both protein and mRNA targets in the same section we have established a semi-automated workflow for sequentially detecting protein and mRNA targets chromogenically on the BOND RX research stainer combining AP/Fast Red detection (RNAscope/Basescope in situ hybridization) with HRP/Green detection (Immunohistochemistry) to establish target mRNA expression in the context of cell lineage detection.

 

Digital Histopathology Deployment in GSK Non-Clinical Histology Facility

Elena Miranda, PhD
Director, Non-Clinical Histology at GSK

Currently, the Pathology community examines relatively small numbers of digital images of H&E stained histological sections for internal consultation and peer review harmonization. In the Non-Clinical Histology team, we focus on a new digital histopathology imaging workflow that will define the standards for histopathology in drug discovery/development for the wider GSK scientific community and expectations for external collaborators. By consolidating metadata standards, governance and data entry systems, the digital histopathology imaging workflow will enable increased application of quantitative image analytic approaches like artificial intelligence to clinical and nonclinical biomarker assays.

For Research Use Only. Not for use in diagnostic procedures


El contenido de Leica Biosystems Knowledge Pathway está sujeto a las condiciones de uso del sitio web de Leica Biosystems, disponibles en: Aviso legal.. El contenido, incluidos los webinars o seminarios web, los recursos de formación y los materiales relacionados, está destinado a proporcionar información general sobre temas concretos de interés para los profesionales de la salud y no está destinado a ser, ni debe interpretarse como asesoramiento médico, normativo o jurídico. Los puntos de vista y opiniones expresados en cualquier contenido de terceros reflejan los puntos de vista y opiniones personales de los ponentes/autores y no representan ni reflejan necesariamente los puntos de vista ni opiniones de Leica Biosystems, sus empleados o sus agentes. Cualquier enlace incluido en el contenido que proporcione acceso a recursos o contenido de terceros se proporciona únicamente por comodidad.

Para el uso de cualquier producto, debe consultarse la documentación correspondiente del producto, incluidas las guías de información, los prospectos y los manuales de funcionamiento.

Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.