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The Aperio Image Analysis IHC menu provides a broad range of solutions for quantification of single and multiplex tissue staining. Flexible algorithms are easily optimized for diverse brightfield chromogens, enabling you to customize the analysis to match your unique research needs. Accurately identify protein biomarker expression at the tissue, cellular or subcellular level.
Aperio RUO (Research Use Only) Image Analysis Algorithms have been validated by Leica Biosystems for use with .svs images from Aperio AT2, Aperio CS2, and Aperio VERSA scanners. Use of Aperio RUO Algorithms with other available scanners has not been validated, and Leica Biosystems cannot train or support customers in use of Aperio RUO Algorithms with images from these scanners.
Identify staining specific to the cell membrane, while eliminating non-specific staining in other subcellular compartments.
Quantify the intensity and amount of staining in cell nuclei, while including only cells of interest based on nuclear morphology and size.
Measure staining located within the cell cytoplasm, while excluding non-specific staining in the membrane or nucleus.
Detect and count objects of interest, such as circulating tumor cells (CTC) or micrometastases, based on color and size.
Automatically detect and measure microvessls, and quantify overall density of vasculature in tissue.
Separate color channels in multiplex stained images, to identify location, area and intensity of each stain.
Determine location of multiple stains, and automatically quantify colocalization of stains across tissue.
What is Digital Pathology?
Four Tips for Digital Pathology Slide Scanning
Immunohistochemistry: An Overview
