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Ki-67 IHC digital slide of invasive breast carcinoma (left) with the DiaKwant™ Ki-67 AI heatmap (right) showing where proliferation concentrates, from low (blue) to high (red), with the three hotspots outlined.

DiaKwant™ Ki-67 Breast

Three hotspots, found for you. Zero click, zero annotation, zero manual counting: when you open the slide in Aperio HALO AP Software, DiaKwant™ AI has already framed the invasive breast carcinoma, classified each tumor nucleus and computed the global Ki-67 score and three hotspot scores, each on at least 1,000 tumor cells. A heatmap shows where proliferation concentrates across the tumor, so the score stays traceable to the tissue. You stay in control. The AI handles the count.

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Feature 01 Ki-67
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Region detection and counting are performed automatically

DiaKwant™ AI selects the invasive tumor on the entire slide, excludes positive lymphocytes from analysis, and locates three hotspots, shown as bounding boxes, where the proportion of Ki-67 positive tumor nuclei is highest. Each holds at least 1,000 tumor cells.

Ki-67 heatmap across the invasive tumor, with the three hotspots outlined in boxes.

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Quantify Ki-67

A global score on the entire invasive area and a score for each of hotspots 1, 2 and 3. The AI handles the count you would do manually on the entire slide.

DiaKwant™ Ki-67 results panel: hotspots score, global score and the score of each hotspot.

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See where proliferation concentrates

A Ki-67 heatmap across the invasive tumor. Switch it on and off to see the tissue underneath.

DiaKwant™ Ki-67 in Aperio HALO AP Software: Ki-67 heatmap and hotspots with the results panel.

DiaKwant Ki-67 Demonstration in Aperio HALO AP Image Management System

DiaKwant Ki-67 is a DiaDeep algorithm integrated within the Aperio HALO AP digital pathology platform to deliver accurate Ki-67 quantification. It automatically analyzes the whole slide to calculate a global Ki-67 index across all invasive tumor areas and to identify the three hotspots with the highest proliferation index, each containing at least 1,000 detected tumor cells, with no manual annotation or selection of invasive regions required from the pathologist.

After selecting a Ki-67 slide, the pathologist can review the tissue in detail. A heatmap overlay highlights the Ki-67 index across the invasive tumor. The heatmap can be switched on or off at any time, allowing the pathologist to compare the algorithm's output with the underlying tissue morphology.

At the end of the analysis, the pathologist obtains:

  • Ki-67 Hotspots Score
  • The global Ki-67 index across all invasive tumor areas.
  • The individual Ki-67 index of hotspots 1, 2, and 3.
  • A heatmap of the Ki-67 index across the invasive tumor.

DiaKwant also offers automated quantification for PD-L1, HER2, ER, PR, and mitosis.

Specifications

Algorithm DiaKwant Ki-67 Breast
Supported image formats SVS, DICOM
Deployment cloud (Software as a Service) or on-premise
Outputs

Scores:

  • Global Ki-67 score over the whole invasive area
  • Hotspot 1, 2 and 3 scores (at least 1,000 tumor cells per hotspot), heatmap

 
Regulatory status Research Use Only (RUO)