Red Counterstain is designed to be used in conjunction with BOND Polymer Refine HRP Plex Detection kit (DS9914) and our chromogen reagents. This counterstain is a fast nuclear red stain which provides excellent contrast against a variety of chromogens, especially when used with the Blue Chromogen (DC9896) and Green Chromogen (DC9913). Red Counterstain is supplied in a volume of 15 mL.

BOND Polymer Refine Red Detection is an IVD labeled red detection system for the automated BOND system. BOND Polymer Refine Red Detection is biotin-free, utilizing alkaline phosphatase (AP)-linked compact polymers to provide enhanced tissue penetration and unsurpassed reagent sensitivity. It contains post primary, polymer reagent, Fast Red chromogen, and hematoxylin counterstain and is supplied in a convenient, ready-to-use format....

Slide 1 Hello, hello; thank you so much for that introduction. Let's get started. Oh, I'm sorry, I just need to hit this next button here. So my name is Mark Lawson. Thank you for joining us in this webinar. I'm here to present BOND RX tips, tricks, and optimization. It's going to be a user guide from the BOND RX and chromogenic multiplexing in the research application. Slide 2 So, my name is Mark Lawson. I'm an application specialist on the Life Sciences team at Leica Biosystems I provide technical support for the Life Sciences portfolio, including but not limited to the BOND RX, the BOND RXm, and a wide array of reagents. So I've worked in the histology field for about 15 years and in both clinical and research spaces. I started off as a histotechnologist and worked my way up...

Biomarkers AE1/AE3 (1) AMACR (2) BCL6 (1) CD163 (2) CD20 (2) CD3 (9) CD4 (5) CD68 (5) CD8 (10) CDX2 (2) CK20 (5) Ck5 (4) ...

Producing an H&E stained slide is a process. It starts way before the slide is loaded on a stainer or moved down through containers by hand. Producing quality, consistent and reproducible H&E stained slides is a process as well as a lesson in...

Immunohistochemistry (IHC) is used in histology to detect the presence of specific protein markers that can assist with accurate tumor classification and diagnosis. This article provides an overview and the basic steps used to create an optimal IHC stain.

Mucins are a part of a complex group called carbohydrates. Mucins are mucopolysaccharides; they are important in cell growth as they help regulate the flow of nutrients between capillaries and cells and are known as “The Glue of Life”.

Special stains" are processes that generally employ a dye or chemical that has an affinity for the particular tissue component that is to be demonstrated. This article includes the processes and protocols necessary to demonstrate connective tissue.

Though the H&E stain is a relatively simple stain to perform, there are a variety of artifacts that can interfere with a good stain. Artifacts can be attributed to a variety of causes.