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How to Create a High-Quality H&E Staining Protocol

Andrew Lisowski, M.S., HTL (ASCP)

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The Hematoxylin and Eosin stain (H&E) is the most widely used stain for histopathological analysis.

Even though the H&E stain is a relatively simple stain to perform, there are a variety of factors that can interfere with the staining process. The maintenance of consistent, high-quality H&E stains is a fundamental requirement in any histopathology related project. Troubleshooting H&E stains can be difficult and frustrating. Our objective is to highlight some of the most common mistakes when choosing staining reagents and creating protocols. By minimizing them, we can deliver high quality slides in the shortest possible time to improve the outcome of translational research projects.

For Research Use Only. Not for use in diagnostic procedures.

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About the presenter

Andrew Lisowski, M.S., HTL (ASCP)

Andrew Lisowski has almost 30 years of experience in histology and histotechnology. He attended veterinary school and earned his master’s degree in molecular biology. Andrew worked in histology, IHC and ISH labs, cell culture lab, performed in-vitro and in-vivo toxicology assays and was a member of a necropsy team. He worked for pharmaceutical companies, medical school and founded his own molecular and histology firms.