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Chromogenic Co-detection of Protein and mRNA Targets in Tissues using the BOND RX Fully Automated Research Stainer

Bradley Spencer-Dene
Bradley Spencer-Dene Subject Matter Expert, Next Gen Histology at GSK

Co-detection of more than one target in Formalin-Fixed Paraffin Embedded samples is an extremely useful application and one that is increasingly required in modern histopathology labs.

In order to detect both protein and mRNA targets in the same section, Bradley Spencer-Dene at GSK, has established a fully-automated workflow for sequentially detecting protein and mRNA targets chromogenically on the BOND RX research stainer combining AP/Fast Red detection (RNAscope/Basescope in situ hybridization) with HRP/Green detection (Immunohistochemistry) to establish target mRNA expression in the context of cell lineage detection.

For Research Use Only. Not for use in diagnostic procedures.  

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