4 result(s) for 'Fluorescent'
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DAPI Counterstain is used as a counterstain for chromosomes. The counterstain consists of DAPI and antifade, which helps preserving the fluorescence signal. DAPI binds strongly to DNA, particularly to AT rich regions. It is used in the staining step of fluorescence in situ hybridization (FISH) on interphase nuclei and metaphase chromosomes. Counterstain Diluent is an antifade solution, which helps preserving the fluorescence signal. It can be use...
Fluorescent in situ hybridization (FISH) identifies or labels target genomic sequences so that their location can be studied. DNA sequences from appropriate chromosome specific probes are first labeled with reporter molecules. The labeled DNA probe is then hybridized to the metaphase chromosomes or interphase nuclei on a slide. After washing, the specimen is screened for the reporter molecules by fluorescence microscopy.For use on metaphase and i...
The t(12;21), a cryptic translocation rarely observed by conventional cytogenetics, was first identified by fluorescence in situ hybridization (FISH). In ALL blasts, this translocation fuses the 5' part of the ETV6 (previously known as TEL) gene with almost the entire RUNX1 (previously known as AML) (CBFA2) gene, producing the chimeric transcript ETV6-CBFA2. The t(12;21) (p13;q22) has also been identified as the most frequent chromosomal abnormal...
Well-differentiated liposarcoma/atypical lipomatous tumor and dedifferentiated liposarcoma are among the most common malignant soft tissue tumors presented in older adults. These tumors can be difficult to distinguish from benign lipomatous neoplasms and other high-grade sarcomas. Amplification of the MDM2 gene has been identified in lipomatous neoplasms. The use of fluorescence in situ hybridization in identifying MDM2 amplification has made the...