Fundamentals of H&E Staining
Overview
H&E staining is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen. H&E is a fast and relatively inexpensive method of assessing tissue morphology.
Learning Objectives
- What is “Routine Staining”
- Histology routine workflow
- Steps leading up to H&E staining
- Proper techniques and common mistakes in
- Processing
- Microtomy
- Proper techniques and common mistakes in
- Science of H&E staining
- Staining procedure methods
- Staining methods
- Staining dyes and ancillaries
- Examples of staining protocols
- Standard
- Using xylene substitutes
- For frozen sectioning
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