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  • Robin Fitzl ,
    Sr. Field Applications Specialist - Leica Biosystems

Tissue Preparation: An essential element for good quality staining of a tissue specimen is thorough fixation and subsequent tissue processing. 

  • Julia Jones ,
    Scientific manager, Histopathology/ISH Core Facility, Cancer Research UK Cambridge Institute, University of Cambridge

Learn more from subject-matter experts from the Cancer Research UK Cambridge Institute, who will share results using chromogenic methods such as integrated co-detection workflow for RNAscope in situ hybridization assays.

Learn more about Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments – NanoString and Leica Biosystems technology combined: A user experience

COVID-19 is a complex multiphase disease in which the immune system causes disease exacerbation in some patients. This talk covers how digital pathology and tissue multiplexing techniques can be combined to effectively investigate the pathophysiology of SARS-CoV-2 to better inform the optimal selection and scheduling of therapeutic approaches.

  • Prof. Aldo Scarpa ,
    EPC President Director of Pathology University of Verona Director ARC-NET: Center for Applied Research on Cancer
  • Dr. Rita Lawlor ,
    Ph.D., CIPP/E, CIPM Fellow of Information Privacy (IAPP)

At the 53rd Meeting of the European Pancreatic Club, Leica Biosystems sponsored interesting short talks from Prof Aldo Scarpa, President of EPC, and Dr Rita Lawlor on "Multiplexing Immunohistochemistry for Applied Research", and “Digital imaging for biobanking and artificial intelligence”.

RNAscope detects RNA in any tissue type and is becoming a widely used technique in research laboratories. Dr. Carossino introduces IHC and ISH  methodologies and platforms, as well as the advantages of automating these techniques with respect to RNAscope and automation with Leica Biosystems BOND RXM.  

  • John Stock ,
    Senior Technical Support Specialist, Leica Biosystems

Processing multiple specimens and performing serial cuts can result in high section numbers. Nevertheless, reproducibility can't be compromised. Permanent attention to detail and many hours in front of the cryostat can be exhausting for the user.  

At the Multiplex User Meeting hosted via LabRoots, Helen Snyder, PhD delivered a live session detailing Cell IDx’s UltraPlex chromogenic multiplex IHC technology. Their chromogenic detection system of 3-4 markers simultaneously is developed to run on BOND RX autostainer in 4-5 hours,  providing maximal information on small tissue samples for biomarker discovery. 

There is an ever-increasing demand to derive more information from limited research samples. Establishing expression profiles of the tumor microenvironment can be achieved via multiplex staining now with chromogenic detection. Discover how the new features of the Leica Biosystems BOND research system and new assays combine to support automated 6-plex chromogenic multiplexing.