Slide 1 Hello, hello; thank you so much for that introduction. Let's get started. Oh, I'm sorry, I just need to hit this next button here. So my name is Mark Lawson. Thank you for joining us in this webinar. I'm here to present BOND RX tips, tricks, and optimization. It's going to be a user guide from the BOND RX and chromogenic multiplexing in the research application. Slide 2 So, my name is Mark Lawson. I'm an application specialist on the Life Sciences team at Leica Biosystems I provide technical support for the Life Sciences portfolio, including but not limited to the BOND RX, the BOND RXm, and a wide array of reagents. So I've worked in the histology field for about 15 years and in both clinical and research spaces. I started off as a histotechnologist and worked my way up...

DESCRIPTION: Chromogenic multiplex IHC kit for BOND RX - human PD-L1, CD8, PanCK SIZE: 10 slides PRODUCT OVERVIEW: Kit for chromogenic multiplex IHC staining of 10 slides plus a single negative control slide on the BOND RX with V7.0 software. 1° Antibody Clone 1° Tag 2° Antibody Chromogen PD-L1 73-10 UT014 Anti-UT014-HRP Leica Blue CD8 EPR10640(2) UT015 Anti-UT015-AP Leica Refine Red PanCK AE1/AE3 UT016 Anti-UT016-HRP Yellow Leica Ref...

DESCRIPTION: Chromogenic multiplex IHC kit for BOND RX - human CD4, CD8, PanCK SIZE: 10 slides PRODUCT OVERVIEW: Kit for chromogenic multiplex IHC staining of 10 slides plus a single negative control slide on the BOND RX with V7.0 software. 1° Antibody Clone 1° Tag 2° Antibody Chromogen CD4 EPR6855 UT014 Anti-UT014-HRP Leica Blue CD8 EPR10640(2) UT015 Anti-UT015-AP Leica Refine Red PanCK AE1/AE3 UT016 Anti-UT016-HRP Yellow Leica Refin...

DESCRIPTION: Chromogenic multiplex IHC kit for BOND RX - human CD68, CD163, PD-L1 SIZE: 10 slides PRODUCT OVERVIEW: Kit for chromogenic multiplex IHC staining of 10 slides plus a single negative control slide on the BOND RX with V7.0 software. 1° Antibody Clone 1° Tag 2° Antibody Chromogen CD8 C68/684 UT021 Anti-UT021-AP Leica Refine Red CD163 EPR19518 UT015 Anti-UT015-HRP Yellow PD-L1 73-10 UT014 Anti-UT014-HRP Leica Blue Leica Refin...

Learn more about multiplexing and explore these educational videos and infographics to understand how to choose chromogen colors for multiplex, as well as the different detection systems available for multiplex assays. 

Immunohistochemistry (IHC) detects proteins of interest within whole tissue sections, keeping cellular organization intact.

Blue Chromogen is designed to be used in conjunction with DS9914 BOND Polymer Refine HRP Plex Detection kit. This chromogen provides a vibrant blue color and is suitable for use with aqueous mounting media. Our Blue Chromogen provides brilliant contrast against other chromogens and counterstains including hematoxylin.  Take advantage of on-board chromogen mixing on BOND instruments, not to mention the fully automated staining opportunities using ...

Green Chromogen is designed to be used in conjunction with DS9914 BOND Polymer Refine HRP Plex Detection kit. This chromogen provides an intense green color and is suitable for use with permanent mounting media such as our CV Ultra mounting media. Our Green Chromogen can be used to provide striking contrast against a multitude of chromogen and counterstain staining combinations. Take advantage of on-board chromogen mixing on BOND instruments, not...

Multiplexing is an important tool for simultaneous detection of multiple markers within a single tissue section. These educational infographics highlight the 5 key reasons to multiplex, as well as the main considerations for choosing chromogenic or immunofluorescent detection.