This article describes a system for embedding of tissues for the preparation of frozen sections. The system is easy to learn and offers many advantages over conventional methods, including speed, high precision and predictability, and reduced tissue wastage.

Slide 1 Welcome! Slide 2 Intro slide Slide 3 It's nice to meet you! Welcome to the Histology Hacks in Cryosectioning webinar. I am Dr. Marla Rivera-Oliver, and I am very excited to share today some tips and tricks I've learned during my ten years of experience working on basic and translational research. I am based in the Dallas, Texas, area of the United States, and in my current role, I support customers from North Texas and Oklahoma. I completed a bachelor's in biology from the Interamerican University of Puerto Rico and a doctoral degree in Neurobiology from the University of Puerto Rico. I specialized in the spinal cord injury field and honed my skills in Cryosectioning, imaging, and in vivo electrophysiology. Then, I moved into a post-doctoral position at the Center for D...

In the webinar Cryosectioning 101 for Research, John Stock provided a comprehensive guide on achieving better cryosectioning outcomes. John went on to answer customer questions at the end of the session, that have been compiled for your viewing here.

FSC 22 is a water soluble embedding compound used in frozen sectioning. The compound bonds and encapsulates tissue specimens to the object holder for cryosectioning. It provides excellent sectioning consistency with minimal curling of sections at a working temperature of -20 °C. FSC 22 is available in clear or in light blue for better visualization of small specimens. It is highly recommended for surgical pathology laboratories....

The CryoJane Tape-Transfer System for Cryosectioning makes it easy to produce high-quality frozen sections of difficult tissues. The CryoJane uses adhesive coated slides and adhesive tapes to capture sections instead of using an anti-roll plate or brush. The Leica CryoJane is adaptable to virtually all cryostats on the market. The system achieves frozen sections of paraffin-quality as thin as two microns. The sections are wrinkle-free, uncompress...

The Brain Punch Tissue Set is used for the Palkovits Punch technique, which allows extraction of minute amounts of brain tissue from exact locations in the brain.  A hypodermic  tube with a sharp rim is pressed onto a frozen section on a glass slide.  The tissue under the hole is freed and pulls away with the punch.  It can then be expelled from the punch with the spring loaded expeller by pressing the top of the punch. The Br...

Antigen BackgroundS-100A and S-100B proteins are two members of the S-100 family of proteins. S-100A subtypes are composed of one alpha and one beta chain, whereas S-100B is composed of two beta chains. S-100 protein is reported to be expressed in neuroectodermal tissue, including nerves and melanocytes. Langerhans cells in skin are also reported to express S-100 protein. It is noteworthy that S-100 protein is highly soluble and may be eluted fro...

A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy...

Get tips for better microtomy, flotation & section drying in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.