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Fundamentals of H&E Staining

Andrew Lisowski M.S., HTL (ASCP)

Overview

H&E staining is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen. H&E is a fast and relatively inexpensive method of assessing tissue morphology.

Learning Objectives

  • What is “Routine Staining”
  • Histology routine workflow
  • Steps leading up to H&E staining
    • Proper techniques and common mistakes in
      • Processing
      • Microtomy
  • Science of H&E staining
    • Staining procedure methods
    • Staining methods
    • Staining dyes and ancillaries
  • Examples of staining protocols
    • Standard
    • Using xylene substitutes
    • For frozen sectioning

About the presenter

Andrew Lisowski , M.S., HTL (ASCP)

Andrew Lisowski has almost 30 years of experience in histology and histotechnology. He attended veterinary school and earned his master’s degree in molecular biology. Andrew worked in histology, IHC and ISH labs, cell culture lab, performed in-vitro and in-vivo toxicology assays and was a member of a necropsy team. He worked for pharmaceutical companies, medical school and founded his own molecular and histology firms.

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