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Enhanced Automated Multiplex Capabilities on Leica Biosystems BOND Research System

There is an ever-increasing demand to derive more information from limited samples. Multiplex staining provides a means of visualizing many targets in a single tissue section, with the ability to accurately resolve spatial relationships between targets. It is a vital tool in establishing expression profiles of immune checkpoint markers in the tumor microenvironment. Multiplex staining has historically been a long process, and when done manually is generally performed over 2 or more days, depending on the number of markers and chromogen combinations used. Automated chromogenic multiplexing applications have largely been limited to 2- or 3-plex stains and to conduct more than 3-plex had required a combination of manual steps (e.g. pre-mixing chromogens) incorporated with several processing runs on the instrument. As a result, these workflow inefficiencies often hindered optimal assay design and staining quality.

The Leica Biosystems BOND research system capabilities have been expanded to further support the need for automating chromogenic multiplexing in tissue. Discover how the new features and assays combine to support automated 6-plex chromogenic multiplexing

Learning objectives

  • Discover the multiplex capabilities of Leica Biosystems BOND research system and how it supports chromogenic and fluorescent multiplexing
  • Learn how to utilize the new features of research BOND software to support multiplexing
  • Review optimized examples of how to automate 6-color chromogenic multiplexing on BOND research system


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For research use only. Not for use in diagnostic procedures.

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