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CHROMOGENIC MULTIPLEXING

Chromogenic Multiplexing (multiplex IHC and ISH)

Chromogenic multiplexing provides the ability to look at 3 or more markers on the same slide using brightfield microscopy. Chromogens provide a more stable and permanent result compared to their fluorescent counterparts.

Normal tonsil tissue stained with:

Marker 1 = PD-L1 = LBS Red
Marker 2 = CD68 = LBS DAB
Marker 3 = CD8 = LBS Blue
Marker 4 = Pan-CK – LBS Green
Counterstain = Hematoxylin

Normal human tonsil stained with CyclinD1 red nuclear stain, CD20 brown membranous stain, and hematoxylin counterstain
Cyclin D1 red nuclear stain
CD20 brown membranous stain
Hematoxylin Counterstain

Tissue – Skin- Melanoma stained with:

Marker 1 - PD-L1 - LBS Red
Marker 2 - CD68 - LBS DAB
Marker 3 - CD8 - LBS Blue
Marker 4 - Pan-CK - LBS Green

In human bladder stained with:

Marker 1 - CK20 - Red
Marker 2 - p53 - Brown
Marker 3 - CD44 - Green
Counterstain = Hematoxylin

Adenocarcinoma in human colon stained with:

Marker 1 - CDX2 - LBS Red
Marker 2 - CD3 - LBS Green
Counterstain = Hematoxylin