Aperio FISH Brk/Fus Algorithm – Automated FISH Break-apart & Fusion Analysis

Aperio FISH Brk/Fus Algorithm

The Aperio FISH Brk/Fus Algorithm enables highly-specific and sensitive detection of DNA sequence translocations (break-apart and fusion) in whole slide digital pathology images. It provides standardized results, removing the inter- and intra-user variability that is inherent in manual FISH counts. Aperio FISH Brk/Fus Algorithm has been developed for full interoperability with the Aperio digital pathology solutions for researchers.

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Developed with you in mind

Aperio FISH Brk/Fus Algorithm is designed to evaluate break-apart and fusion events within target gene sequences. Specifically tailored to meet workflow challenges, such as the occurrence of “beads on a string” signals, you can be confident that the algorithm is ready to meet your needs. 

Easy to use

Set-up of the algorithm is performed via an intuitive tuning wizard, so even inexperienced users can quickly and easily create their own custom analysis tool. Visual mark-up is provided at each stage in the process, with live feedback on results as each setting is changed.

Get the most from your samples

Analyze complex patterns of gene sequence translocation precisely and automatically. Up to 7 signals of interest, in addition to nuclear counterstain, can be analyzed on a single slide. Cells are classified into multiple user-defined categories based on number of signals, break-aparts, and fusions.

Flexible analysis

Customizable inputs allow the algorithm to be used flexibly with a variety of probes and applications. Users can exclude cells that are not of interest, and adjust signal detection to exclude staining artefacts, haze, and hot signals that may affect analysis.

Key downloads

 Brochure (pdf)

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Aperio RUO (Research Use Only) Image Analysis Algorithms have been validated by Leica Biosystems for use with .svs images from Aperio AT2, Aperio CS2, and Aperio VERSA scanners. Use of Aperio RUO Algorithms with other available scanners has not been validated, and Leica Biosystems cannot train or support customers in use of Aperio RUO Algorithms with images from these scanners.