Dual probe FISH Amplification Deletion original image
Aperio FISH Amp/Del Algorithm mask on dual probe image. Detected signals (in this case, red & green) are indicated in the corresponding color. Mask colors indicate the scoring category each cell falls into. GREEN = Normal, BLUE = Target probe deletion, GOLD = Equivocal.
Aperio FISH Amp/Del Algorithm Optimization in Aperio ImageScope showing tuning window with live feedback.
Tuning Aperio FISH/Amp Del Algorithm in Aperio ImageScope on a multiplex assay (UroVysion from Abbott Molecular). Mark-up shows detected signals in the corresponding colors (RED = 3cen, GOLD = 9p21, GREEN = 7cen, AQUA = 21cen), as well as masks indicating scoring classification of each cell (RED = Polyploid, TEAL = Other deletion of 9p21, GOLD = Other abnormal).
Aperio FISH Amp/Del Algorithm Per-Cell results showing outputs for individual cells.