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Validating your H&E Staining Systems

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Overview :

In this age of healthcare regulatory standards and compliance, every aspect in diagnostic patient care must be developed, documented, and validated ‘before’ they can be used in testing and diagnoses. Procedures which were once deemed as ‘routine’, as in the routine staining procedure (H&E) which constitutes 95-100% of most hospital initial testing review in histopathology; these procedures including protocol, stains, tissue specimens, and Q.A. monitoring and maintenance, all must go through stringent testing to show they produce reliable, reproducible results. This session will look at every aspect of your routine staining system from the stain dyes used (hematoxylin and eosin), various protocol trials with different staining intensities, the differences in instrumentation and how they perform, specimens being used to validate the stain, and technical review and sign-off by the pathologist.

Learning Objectives :

  1. Create a standard H&E multi-tissue control block
  2. Set up an internal quality assurance schedule
  3. Document and retain the validation procedure for regulatory inspection

Presenter :

Skip Brown has 39 years of experience in the field of Histotechnology, over 30 of which have been at the supervisory and management levels. He has managed some of the most progressive clinical and research institutions such as Kaiser Permanente of Southern California and Northwestern University Pathology Core Facility. His passion is teaching and training and he has been one of the premier educators in the U.S. in lab management and histotechnology. He was recently awarded the “Excellence in Teaching Award” at a National Society for Histotechnology Symposium. Having designed and managed laboratory systems in the past, Skip has always been fascinated by the process of laboratory workflow and how to streamline it.

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