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The H&E Stain – A Lesson on Consistency and Reproducibility


Producing an H&E stained slide is a process. It starts way before the slide is loaded on a stainer or moved down through containers by hand. Producing quality, consistent and reproducible H&E stained slides is a process as well as a lesson in standardization. In this presentation, we will look at the process and learn the standardization necessary to have consistent and reproducible H&E slides.

Learning Objectives:

  1. Review the many factors that influence the quality of an H&E stain.
  2. Describe how an H&E works.
  3. Explain what makes a regressive H&E stain different from a progressive one.
  4. Review what experts suggest in order to maintain consistent and reproducible H&Es.

Carolyn Doan received her ASCP registration in 1969 after completing classes at Georgia State University and St Joseph school of histotechnology in Atlanta, Georgia. For 40 years she shared her passion for histology in research (at Yerkes Primate Research Center), in the clinical world (managing the Pathology Department at Florida Hospital in Orlando and serving on the board of the Florida State Licensure task Force and as president of the Florida State Society for Histotechnology) and in the industry (as a Sales executive, Marketing Manager and North American staining sales specialist). Since her retirement in 2013, she founded Creative Histology Consulting.

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