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Multiplexing is an important tool for simultaneous detection of multiple markers within a single tissue section. These educational infographics highlight the 5 key reasons to multiplex, as well as the main considerations for choosing chromogenic or immunofluorescent detection.
San Diego, CA – November 8, 2022. Leica Biosystems, a technology leader in automated staining and brightfield and fluorescent imaging, has acquired Cell IDx, Inc., a leader in multiplexed tissue profiling. Founded in 2012 and headquartered in San Diego, California, Cell IDx provides multiplex staining panels, tissue staining, and imaging and analysis services.
Cell IDx’s UltraPlex technology allows simultaneous detection of multiple biomarkers in many immunoassays through fluorescent and chromogenic multiplex immunohistochemistry (“IHC”) staining technology, and automated chromogenic detection of multiple markers on Leica Biosystems’ BOND RX fully automated research stainer. Together, UltraPlex and BOND RX with high throughput imaging allows researchers to detect three or more ...
Incorporation and Automation of RNAscope Technology into your COVID-19 Viral Pathogenesis Research. Research Use Only, Not for Clinical Diagnostic Procedure
For Research Use Only. Not For Diagnostic Use. Incorporation of the highly sensitive and specific RNAscope technology into your COVID-19 Viral Pathogenesis Research Jyoti Sheldon Applications Research Use Only, Not for Clinical Diagnostic Procedure
Agenda RNAscope technology overview COVID-19 Offerings COVID-19 Publications Research Use Only, Not for Clinical Diagnostic Procedure
RNAscope®In Situ Hybridization (ISH) Technology A hybridization-based signal amplification system with a unique probe design - Simultaneous signal amplification and background suppression Amplification Target-specific binding A specific and sensit...
Fluorescent in situ hybridization (FISH) identifies or labels target genomic sequences so that their location can be studied. DNA sequences from appropriate chromosome specific probes are first labeled with reporter molecules. The labeled DNA probe is then hybridized to the metaphase chromosomes or interphase nuclei on a slide. After washing, the specimen is screened for the reporter molecules by fluorescence microscopy.For use on metaphase and i...
Phenotyping Tumor Microenvironment Using DNA Barcoded Multiplex Technology Chifei Sun GSK IVIVTBIBCellular Biomarkers Collegeville, PA
I have no conflict of interests with Leica Biosystems, Ultivue and Indica Labs. – All the materials used in this presentation are for educational purpose only. – The human biological samples were sourced ethically, and their research use was in accord with the terms of the informed consents under an IRB/EC approved protocol. Statement 9/25/2020 2
Understand the complexity of tumor microenvironment and its importance in drug development – Introduce the Ultivue DNA barcoding and exchange technology for multiplex immunofluorescence – Highlight the processes of image fusion, spatial analysis and colocalization of markers on individual cells Learning...
The Power of Digital Pathology in Research
1. Leica Biosystems Proprietary Information. For Research Use Only. Not For Use in Diagnostic The Power of Digital Pathology In Research. Caitríona Lyons PhD.
2. Introduction • Caitríona Lyons PhD • Based in Dublin, Ireland • Current Role: • Product Manager Image Analysis, Leica Biosystems • Previous Roles: • Product Owner Image Analysis, Leica Biosystems • Image Analysis Specialist, Leica Biosystems • Education • BSc (Hons) Biomedical Science • PhD Medicine and Health • Postdoc Immunology
3. Agenda • History of Pathology • What is Digital Pathology • Power of Digital Pathology • Leica Biosystems Role in Digital Pathology • The Future of Digital Pathology • Final Thoughts 3 SCAN MANAGE ANALYZE
4. History of Digital Pathology • Patholog...
Imagine the possibilities for your Chromogenic Multiplex with Leica Biosystems and Cell IDx
To accelerate the discovery journey in cancer research, Leica Biosystems has partnered with Cell IDx, a technology leader in multiplexed cellular immunodiagnostics, developing highly sensitive and specific reagents for research and clinical immunohistochemistry to meet the needs of precision medicine.
Cell IDx technology allows the automated detection of multiple markers on the BOND RX staining system. The technology uses primary antibodies from any species, employing a single antigen retrieval step that allows detection of three chromogen stains in 4.5 hours and four chromogen stains in 6 hours. The process is simple, fast, and fully automated on the BOND RX platform and Aperio Brightfi...
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Welcome to the Histology Hacks in Cryosectioning webinar.
I am Dr. Marla Rivera-Oliver, and I am very excited to share today some tips and tricks I've learned during my ten years of experience working on basic and translational research. I am based in the Dallas, Texas, area of the United States, and in my current role, I support customers from North Texas and Oklahoma. I completed a bachelor's in biology from the Interamerican University of Puerto Rico and a doctoral degree in Neurobiology from the University of Puerto Rico. I specialized in the spinal cord injury field and honed my skills in Cryosectioning, imaging, and in vivo electrophysiology. Then, I moved into a post-doctoral position at the Center for D...
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Hello, hello; thank you so much for that introduction. Let's get started. Oh, I'm sorry, I just need to hit this next button here. So my name is Mark Lawson.
Thank you for joining us in this webinar. I'm here to present BOND RX tips, tricks, and optimization. It's going to be a user guide from the BOND RX and chromogenic multiplexing in the research application.
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So, my name is Mark Lawson. I'm an application specialist on the Life Sciences team at Leica Biosystems I provide technical support for the Life Sciences portfolio, including but not limited to the BOND RX, the BOND RXm, and a wide array of reagents. So I've worked in the histology field for about 15 years and in both clinical and research spaces. I started off as a histotechnologist and worked my way up...