19 result(s) for 'Colon'
1 - 10 of 19 results for 'Colon'
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Antigen Background
MLH1, a mismatch repair protein involved in maintaining the integrity of genetic information, alongside MSH2, MSH6 and PMS2. During DNA replication, strand misalignment can occur resulting in alterations to microsatellite repeats, often referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been linked to human carcinogenesis. Mutations in the MLH1 gene have been reported to be found in some ...
Deletions affecting the long arm of chromosome 6 (6q) involving band 6q21 are among the most commonly observed chromosomal aberrations in lymphoid malignancies and have been identified as adverse prognostic factor in subsets of tumors. Amplification of MYC (8q24) has been described in many types of solid tumors, such as breast, cervical and colon cancers, as well as in myeloma, non-Hodgkin's lymphoma, gastric adenocarcinomas and ovarian cancer. T...
Aurora kinase A (AURKA) gene amplification has been detected in approximately 12% of primary breast tumors, as well as in bladder, ovarian, colon, prostate, neuroblastoma and cervical cancer cell lines. The AURKA (20q13)/20q11 probe is designed to detect copy numbers of the AURKA gene region at region 20q13. The AURKA (20q13) FISH probe is optimized to detect copy numbers of the AURKA gene region at region 20q13. The 20q11 specific DNA probe is i...
Antigen Background
Human mismatch repair protein 2 (MSH2) is involved in the initial recognition of mismatched nucleotides during the post replication mismatch repair process. Therefore, the loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple mutations required for multistage carcinogenesis. MSH2 is reported to be expressed in the nuclei of cells from a variety of tissues includ...
The ERBB2 (or HER2) gene encodes a receptor tyrosine kinase involved in growth factor signaling. Overexpression of this gene is seen in about 20% of invasive breast cancers. ERBB2 gene amplification is a permanent genetic change that results in this continuous overexpression of ERBB2. ERBB2 amplification is also observed in a variety of other tumors, such as gastric, prostate, lung, colon and ovary carcinoma. The ERBB2 (17q12) FISH probe is optim...
Antigen Background
Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosomal condensation, DNA replication, transcription, recombination and segregation at mitosis. Human Topoisomerase I is a 100 kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single-stranded nick which is then re-ligated at the end of the reaction. It has been shown that the enzyme is lo...
Slide 1
Welcome
Slide 2
Slide 3
Discuss Pre-Analytics and the impact of improper fixation and artifacts on tissue processing
Discuss Fixation and the impact of incomplete fixation on tissue processing
Describe the impact of improper Prosection
Determine satisfactory Processing of samples
Explain why routine Maintenance is a critical success factor to proper tissue processing
Slide 4
This slide has two examples of what the ideal sections in a perfect world look like under the scope. The skin section on the left is clean looking with the Eosin staining the components of the dermis with different shades. The colon section on the right is crisp with well-defined nuclei and cilia. There is no background staining or muddiness to the stain.
Slide 5
One artifact seen the lab that is n...
One of the most fundamentally critical elements of diagnostic histopathology is first the ability to suspend all cellular activity in tissue and prevent degradation, and secondly to process that specimen in a manner that facilitates subsequent steps such as...
Slide 1
Hello, hello; thank you so much for that introduction. Let's get started. Oh, I'm sorry, I just need to hit this next button here. So my name is Mark Lawson.
Thank you for joining us in this webinar. I'm here to present BOND RX tips, tricks, and optimization. It's going to be a user guide from the BOND RX and chromogenic multiplexing in the research application.
Slide 2
So, my name is Mark Lawson. I'm an application specialist on the Life Sciences team at Leica Biosystems I provide technical support for the Life Sciences portfolio, including but not limited to the BOND RX, the BOND RXm, and a wide array of reagents. So I've worked in the histology field for about 15 years and in both clinical and research spaces. I started off as a histotechnologist and worked my way up...
Biomarkers
AE1/AE3 (1)
AMACR (2)
BCL6 (1)
CD163 (2)
CD20 (2)
CD3 (9)
CD4 (5)
CD68 (5)
CD8 (10)
CDX2 (2)
CK20 (5)
Ck5 (4)
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