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Paper Cryoembedding

The article describes a unique method of face-down tissue embedding for frozen section in freezing-temperature well bars.

Tissue is first placed face down in a precise position on a piece of lens paper. The tissue is then transferred in the exact position to an embedding well.

The block is completed while the tissue remains face down on the paper. The paper is removed during the initial trimming of the block, leaving the tissue in its original position and orientation.

The technique accomplishes precise embedding of extremely delicate and deformable tissues and arrangements of tissues.

We believe this technique will facilitate a variety of difficult embedding tasks in areas of surgical pathology, Mohs surgery, and research settings
(The J Histotechnol 26:173, 2003).

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