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Leica SM2400 Materials Testing

Leica SM2400 Sliding Microtome for Biological Applications

Sliding Microtomes

How can I avoid chatter?

Problem: Clearance angle is too big Systematically decrease the clearance angle until the optimum adjustment is obtained.   Problem: Unsuitable knife/blade profile Try a knife with a different profile. The high profile blade might be too big, try using a low profile blade or use a different brand. The blade might be too thin, try using a high profile blade or use a different brand.      Problem: Blade holder pressure plate is damaged or wax is built-up Try a new blade holder or use a different part of the blade holder. Remove and clean the rear of the pressure plate.   Problem: Insufficient clamping of specimen and/or knife Check if all levers are locked and screws are tightened.   Note: The reason might be an application issue, not a microtome problem Consider the following factors that can influence sectioning: Infiltration Dehydration Decalcification Paraffin age Paraffin purity   Problem: Table-top stability Try to steady the microtome or replace the table if it is not sufficiently sturdy.   Problem: Sectioning speed too high Work at a slower sectioning speed. Thin sections require a slow cutting speed.  

How can I stop thick/thin sections and in extreme cases, sections being completely skipped?

Problem: Insufficient clamping of specimen and/or knife Check that all levers are locked and screws are tightened. Ensure the cassette isn't under filled or over filled (excess of paraffin on the outside of the cassette has to be removed before clamping). Ensure the correct clamping system is used (standard clamp/UCC).   Problem: The clearance  angle is too small/big. Systematically decrease/increase the clearance angle until the optimum angle is found.   Problem: Blunt knife/blade Use a different part of the knife or blade edge. Use a new knife or blade.   Problem: Excess oil on the knife Remove the oil with soft paper or Q-tip.   Problem: The specimen block is too large or too hard If possible trim the specimen. Soften the block by using Osteosoft® or soak the block with iced water. Try a knife with a different profile (d-profile instead of c-profile).  

There is no specimen feed and I obtain no section. What can I do?

Problem: Upper feed limit reached Turn the coarse feed wheel clockwise to lower the specimen.   Problem: User may accidentally be holding coarse feed wheel while sectioning. Stop holding the coarse feed wheel.   Problem: The coarse feed wheel cannot rotate freely. Remove any obstruction. Problem: Internal mechanism (gear) might be defective. Contact your service representative for replacement of coarse advance mechanism.